This mouse IgM monoclonal antibody recognizes the amino-acid sequence corresponding to the 3'-UGA codon region of human cluster of differentiation antignen 4 (CD4).
Eukaryotic genes end with one of three stop codons, UGA, UAA, or UAG. Of these three, UGA is unique. In addition to functioning as a stop codon, UGA is also the codon for the twenty-first amino acid, selenocysteine. The biological bases for the dual function of the UGA codon are not entirely clear. Selenocysteine plays a critical role in physiology, and perturbations of selenium intake and metabolism can have disastrous consequences. CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus.
From the laboratory of Ethan W. Taylor, PhD, University of Georgia.
Part of The Investigator's Annexe program.
|Antigen:||RFLPADQMNVADPQPLASCS of human CD4|
|Clone Name:||8A5, also known as Antibody 025|
|Reactivity:||Human (possibly others)|
|Purification Method:||Not purified|
|Buffer:||Cell culture supernatant|
|Tested Applications:||Use undiluted or up to 1:300. Dilute in PBS or other compatible medium as required by specific experiment. For ELISA: 10-25 ug/ml (Jurkat, H9, HeLa). For IF: 5-12 ug/ml|
|Storage:||-80C (long term)|
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