Anti-eEF3 Antibody

This rabbit polyclonal antibody was generated against N-terminal 6xHis-tagged full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 3 (eEF3).

Highlights

  • Reacts with Saccharomyces cerevisiae eukaryotic translation elongation factor 3 (eEF3)
  • Suitable for Western Blot applications

Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 3 is required for the ATP-dependent release of deacylated tRNA from the ribosomal E-site during protein biosynthesis. eEF3 stimulates the eEF1A-dependent binding of aminoacyl-tRNA to the ribosomal A-site, which has reduced affinity for tRNA as long as the E-site is occupied.

From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ED7003
Anti-eEF3 Antibody
Lyophilized serum In stock
Regular Price:$376.00
On Sale:
Specifications

Product Type: Antibody
Accession ID: NP_014384
Antigen: Saccharomyces cerevisiaeeukaryotic Translation Elongation Factor 3 (eEF3)
Molecular Weight: 116 kDa
Clonality: Polyclonal
Reactivity: Saccharomyces cerevisiae, other fungi
Immunogen: N-terminal 6xHis-tagged full length protein
Species Immunized: Rabbit
Buffer: Lyophilized whole serum
Tested Applications: WB (1:10,000)
Storage: 4C
Shipped: Ambient temperature

Documentation

Protocol Notes

Reconstitute in 25uL water.

Provider
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
References
  1. Anand M, Chakraburtty K, Marton MJ, Hinnebusch AG, Kinzy TG. Functional interactions between yeast translation eukaryotic elongation factor (eEF) 1A and eEF3. J Biol Chem. 2003 Feb 28;278(9):6985-91. Epub 2002 Dec 18.
  2. Shamsuzzaman M, Bommakanti A, Zapinsky A, Rahman N, Pascual C, Lindahl L. Analysis of cell cycle parameters during the transition from unhindered growth to ribosomal and translational stress conditions. PLoS One. 2017 Oct 13;12(10):e0186494. View Article

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