Anti-eEF2 Antibody
This rabbit polyclonal antibody was generated against C-terminal 6xHis-tagged full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 2 (eEF2).
Highlights
- Specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 2 (eEF2) - Does NOT recognize human eEF2
- Suitable for Western Blot applications
Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 2 catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. eEF2 catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
This rabbit polyclonal antibody was generated against C-terminal 6xHis-tagged full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 2 (eEF2).
Highlights
- Specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 2 (eEF2) - Does NOT recognize human eEF2
- Suitable for Western Blot applications
Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 2 catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. eEF2 catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
| Product Type: | Antibody |
| Antigen: | Saccharomyces cerevisiaeeukaryotic Translation Elongation Factor 2 (eEF2) |
| Accession ID: | P13639, NP_014776 |
| Molecular Weight: | 93 kDa |
| Clonality: | Polyclonal |
| Reactivity: | Saccharomyces cerevisiae, other fungi |
| Immunogen: | C-terminal 6xHis-tagged full length protein |
| Species Immunized: | Rabbit |
| Buffer: | Lyophilized whole serum |
| Tested Applications: | WB (1:10,000) |
| Storage: | 4C |
| Shipped: | Ambient temperature |
Protocol Notes
Reconstitute in 25uL water.
NotesDoes not recognize human eEF2.
- Ortiz PA, Kinzy TG. Dominant-negative mutant phenotypes and the regulation of translation elongation factor 2 levels in yeast. Nucleic Acids Res. 2005 Oct 6;33(18):5740-8. Print 2005.
- Wu CC, Zinshteyn B, Wehner KA, Green R. High-Resolution Ribosome Profiling Defines Discrete Ribosome Elongation States and Translational Regulation during Cellular Stress. Mol Cell. 2019 Mar 7;73(5):959-970.e5. View Article
- Schopf FH, Huber EM, Dodt C, Lopez A, Biebl MM, Rutz DA, Mühlhofer M, Richter G, Madl T, Sattler M, Groll M, Buchner J. The Co-chaperone Cns1 and the Recruiter Protein Hgh1 Link Hsp90 to Translation Elongation via Chaperoning Elongation Factor 2. Mol Cell. 2019 Apr 4;74(1):73-87.e8. View Article
- D'Orazio KN, Wu CC, Sinha N, Loll-Krippleber R, Brown GW, Green R. The endonuclease Cue2 cleaves mRNAs at stalled ribosomes during No Go Decay. Elife. 2019 Jun 20;8. View Article
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