Anti-eEF2 Antibody

This rabbit polyclonal antibody was generated against C-terminal 6xHis-tagged full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 2 (eEF2).

Highlights

  • Specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 2 (eEF2) - Does NOT recognize human eEF2
  • Suitable for Western Blot applications

Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 2 catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. eEF2 catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ED7002
Anti-eEF2 Antibody
Lyophilized serum In stock
Regular Price:$256.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Saccharomyces cerevisiaeeukaryotic Translation Elongation Factor 2 (eEF2)
Accession ID: NP_014776
Molecular Weight: 93 kDa
Clonality: Polyclonal
Reactivity: Saccharomyces cerevisiae, other fungi
Immunogen: C-terminal 6xHis-tagged full length protein
Species Immunized: Rabbit
Buffer: Lyophilized whole serum
Tested Applications: WB (1:10,000)
Storage: 4C
Shipped: Ambient temperature

Documentation

Protocol Notes

Reconstitute in 25uL water.

Notes

Does not recognize human eEF2.

Provider
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
References
  1. Ortiz PA, Kinzy TG. Dominant-negative mutant phenotypes and the regulation of translation elongation factor 2 levels in yeast. Nucleic Acids Res. 2005 Oct 6;33(18):5740-8. Print 2005.
  2. Wu CC, Zinshteyn B, Wehner KA, Green R. High-Resolution Ribosome Profiling Defines Discrete Ribosome Elongation States and Translational Regulation during Cellular Stress. Mol Cell. 2019 Mar 7;73(5):959-970.e5. View Article
  3. Schopf FH, Huber EM, Dodt C, Lopez A, Biebl MM, Rutz DA, Mühlhofer M, Richter G, Madl T, Sattler M, Groll M, Buchner J. The Co-chaperone Cns1 and the Recruiter Protein Hgh1 Link Hsp90 to Translation Elongation via Chaperoning Elongation Factor 2. Mol Cell. 2019 Apr 4;74(1):73-87.e8. View Article
  4. D'Orazio KN, Wu CC, Sinha N, Loll-Krippleber R, Brown GW, Green R. The endonuclease Cue2 cleaves mRNAs at stalled ribosomes during No Go Decay. Elife. 2019 Jun 20;8. View Article

If you publish research with this product, please let us know so we can cite your paper.

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