Anti-eEF1A Antibody

This rabbit polyclonal antibody was generated against full length protein and is specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 1A (eEF1A).

Highlights

  • Specific for Saccharomyces cerevisiae eukaryotic translation elongation factor 1A (eEF1A) - Does NOT recognize human eEF1A
  • Suitable for Western Blot applications

Elongation factors are proteins used in translation or protein synthesis during the process of cell cycle and elongation in some cells. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. Specifically, elongation factor 1 alpha is the GTP-binding component of the eukaryotic elongation factor 1 complex (eEF1). In its active GTP-bound form, it binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. The inactive GDP-bound form leaves the ribosome and must be recycled by its guanine nucleotide exchange factor (GEF) (eEF1B subcomplex) before binding another molecule of aminoacyl-tRNA. eEF1A is required for nuclear export of aminoacyl-tRNAs, and may also be involved in translational quality control by targeting cotranslationally damaged proteins to the proteasome. eEF1A also exhibits actin filament-binding and -bundling activities and is involved in cytoskeleton organization.

From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ED7001
Anti-eEF1A Antibody
Lyophilized serum In stock
Regular Price:$256.00
On Sale:
Specifications

Product Type: Antibody
Accession ID: NP_015405
Antigen: Saccharomyces cerevisiaeeukaryotic Translation Elongation Factor 1A (eEF1A)
Molecular Weight: 50 kDa
Clonality: Polyclonal
Reactivity: Saccharomyces cerevisiae, other fungi
Immunogen: Full length protein
Species Immunized: Rabbit
Buffer: Lyophilized whole serum
Tested Applications: WB (1:10,000)
Storage: 4C
Shipped: Ambient temperature

Documentation

Protocol Notes

Reconstitute in 25uL water.

Notes

Does not recognize human eEF1A.

Provider
From the laboratory of Terri Goss Kinzy, PhD, Rutgers University.
References
  1. Carr-Schmid A, Valente L, Loik VI, Williams T, Starita LM, Kinzy TG. Mutations in elongation factor 1beta, a guanine nucleotide exchange factor, enhance translational fidelity. Mol Cell Biol. 1999 Aug;19(8):5257-66.
  2. Jindal S, Ghosh A, Ismail A, Singh N, Komar AA. Role of the uS9/yS16 C-terminal tail in translation initiation and elongation in Saccharomyces cerevisiae. Nucleic Acids Res. 2018 Nov 27. View Article

If you publish research with this product, please let us know so we can cite your paper.

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