Anti-Rhamnogalacturonan-I [CCRC-M125] Antibody (supernatant)

This mouse IgM monoclonal (CCRC-M125) was generated against Rhamnogalacturonan-II/ITC-cBSA (covalent) and recognizes the glycan group of Rhamnogalacturonan I/Arabinogalactan.

Highlights:

  • Reacts with tomato, sycamore and others glycan group of Rhamnogalacturonan I/Arabinogalactan
  • Suitable for ELISA, Immunolabeling and Immunofluorescence applications

Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of a-D-GalpA-(1,2)-a-L-Rhap-(1), and carry diverse side chains that are attached to the Rha residues and are composed largely of arabinosyl and/or galactosyl residues.

From the laboratory of Michael G. Hahn, PhD, University of Georgia

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA818
Anti-Rhamnogalacturonan-I [CCRC-M125] Antibody (supernatant)
Supernatant, 5mL 4-6 weeks
Regular Price:$310.00
On Sale:
Specifications

Product Type: Antibody
Accession ID: CCRC M125
Antigen: Rhamnogalacturonan-I, Galactan, Galactomannan, Xyloglucan
Isotype: IgM
Clonality: Monoclonal
Clone Name: CCRC M125
Reactivity: Tomato, sycamore, and others
Immunogen: Rhamnogalacturonan-II/ITC-cBSA (covalent)
Species Immunized: Mouse
Buffer: Cell culture supernatant, Use undiluted or at 1:10 dilution
Tested Applications: ELISA, Immunolabeling, IF
Storage: <1 month at 4C, >1 month at -80C
Shipped: Cold Packs

Documentation

PDF CCRC-M125 Cross Reactivity Map

Notes

CCRC-M125 binds to an epitope present on several distinct classes of polysaccharides, particularly those from tomato.

These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).

Provider
From the laboratory of Michael G. Hahn, PhD, University of Georgia
References
  1. Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive tool kit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
  2. Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.

If you publish research with this product, please let us know so we can cite your paper.

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