Anti-Rhamnogalacturonan-I [CCRC-M7] Antibody (supernatant)
![Anti-Rhamnogalacturonan I [CCRC-M7] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1035_2001202001263110340.jpg "Anti-Rhamnogalacturonan-I [CCRC-M7] Antibody (supernatant)")
This mouse IgG1 monoclonal (CCRC-M7) was generated against Sycamore Rhamnogalacturonan-I and belongs to the Rhamnogalacturonan-I/Arabinogalactan clade of antibodies and binds to a trimer or larger of beta-(1,6)-Gal.
Highlights:
- Reacts with trimer or larger of beta-(1,6)-Gal.
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of α-D-GalpA-(1,2)-α-L-Rhap-(1), and carry diverse side chains that are attached to the Rha residues and are composed largely of arabinosyl and/or galactosyl residues.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
This mouse IgG1 monoclonal (CCRC-M7) was generated against Sycamore Rhamnogalacturonan-I and belongs to the Rhamnogalacturonan-I/Arabinogalactan clade of antibodies and binds to a trimer or larger of beta-(1,6)-Gal.
Highlights:
- Reacts with trimer or larger of beta-(1,6)-Gal.
- Suitable for ELISA, Immunolabeling and Immunofluorescence applications
Rhamnogalacturonans (RGs) are a family of related cell wall pectic polysaccharides that contain a repeating backbone of α-D-GalpA-(1,2)-α-L-Rhap-(1), and carry diverse side chains that are attached to the Rha residues and are composed largely of arabinosyl and/or galactosyl residues.
From the laboratory of Michael G. Hahn, PhD, University of Georgia.
| Product Type: | Antibody |
| Accession ID: | CCRC-M7 |
| Antigen: | Rhamnogalacturonan I of Sycamore maple (Acer pseudoplatanus) |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | CCRC-M7 |
| Reactivity: | Lettuce, tomato, sycamore, and others |
| Immunogen: | Rhamnogalacturonan-II/ITC-cBSA (covalent) |
| Species Immunized: | Mouse |
| Epitope: | Trimer or larger of beta-(1,6)-Gal, some substitution of single Ara residues is tolerated by CCRC-M7 |
| Buffer: | Cell culture supernatant, Use undiluted or at 1:10 dilution |
| Tested Applications: | ELISA, Immunolabeling, IF |
| Storage: | <1 month at 4C, >1 month at -80C |
| Shipped: | Cold Packs |
The epitope recognized by CCRC-M7 is present on rhamnogalacturonan I, and on soluble and membrane-bound arabinogalactan glycoproteins.
These monoclonal antibodies were developed under the sponsorship of the US National Science Foundation, through award number DBI-0421683. Their use in biomass characterization, study of biomass deconstruction and quantitation was developed under the sponsorship of the US Department of Energy through awards DE-PS02-06ER64304 and DE-AC05-00OR22725 (BioEenergy Science Center).
- J. Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-1,2-linked fucosyl-containing epitope. Plant Physiol.104:699-710, 1994.
- W. Steffan, P. Kovac, P. Albersheim, A. G. Darvill, and M. G. Hahn. Characterization of a monoclonal antibody that recognizes an arabinosylated (1,6)-beta-D-galactan epitope in plant complex carbohydrates. Carbohydr.Res. 275:295-307, 1995.
- Pattathil S, Avci U, Baldwin D, et al. 2010. A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiology 153, 514-525.
- Pattathil S, Avci U, Miller JS, Hahn MG. 2012. Immunological approaches to plant cell wall and biomass characterization: Glycome profiling. In: Himmel M (ed) Biomass Conversion: Methods and Protocols. Springer Science + Business Media, LLC, New York, NY, pp 61-72.
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