This rat IgG1 monoclonal antibody [E8-8C3C10] was generated against 6xHis-tagged fusion protein and recognizes the third extracellular loop (i.e. directed to the C-terminal cleavage fragment) of polycystin-1 (PC1).
Polycystin-1 (PC1) is the gene product of PKD1 that is mutated in human autosomal dominant polycystic Kidney disease. It is required for tubular morphogenesis and maintenance of the kidney and other organs. PC1 is a 11-transmembrane glycoprotein that is cic-autoproteolytically cleaved at juxtamembrane extracellular GPS/GAIN in vivo, resulting a N-terminal cleavage fragment and a C-terminal cleavage fragment in the kidney and other tissues.
|Reactivity:||Human and mouse|
|Immunogen:||6xHis-tagged fusion protein|
|Epitope:||Third extracellular loop of mouse PC1|
|Purification Method:||Protein G Affinity|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB (1ug/mL) and IP(0.1-2ug per 2mg of protein)|
Immunoblot detection of recombinant human or mouse PC1 by E8. E8 can detect full length (FL)PC1 and the C-terminal fragment (CTF).
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