Anti-Polycystin-1 [E8-8C3C10] Antibody

This rat IgG1 monoclonal antibody [E8-8C3C10] was generated against 6xHis-tagged fusion protein and recognizes the third extracellular loop (i.e. directed to the C-terminal cleavage fragment) of polycystin-1 (PC1).


  • Reacts with human and mouse Polycystin-1
  • Suitable for Western Blot and Immunoprecipitation applications

Polycystin-1 (PC1) is the gene product of PKD1 that is mutated in human autosomal dominant polycystic Kidney disease. It is required for tubular morphogenesis and maintenance of the kidney and other organs. PC1 is a 11-transmembrane glycoprotein that is cic-autoproteolytically cleaved at juxtamembrane extracellular GPS/GAIN in vivo, resulting a N-terminal cleavage fragment and a C-terminal cleavage fragment in the kidney and other tissues.

From the laboratories of Feng Qian, PhD, and Terry J. Watnick, MD, University of Maryland, Baltimore

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Anti-Polycystin-1 [E8-8C3C10] Antibody
100ug 1-2 weeks
Regular Price:$385.00
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Product Type: Antibody
Antigen: Polycystin-1
Accession ID: P98161, NP_000287.3
Isotype: IgG1
Clonality: Monoclonal
Clone Name: E8-8C3C10
Reactivity: Human and mouse
Immunogen: 6xHis-tagged fusion protein
Species Immunized: Rat
Epitope: Third extracellular loop of mouse PC1
Purification Method: Protein G Affinity
Buffer: PBS, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1ug/mL) and IP(0.1-2ug per 2mg of protein)
Storage: -20C
Shipped: Cold packs


Western Blot

Immunoblot detection of recombinant human or mouse PC1 by E8. E8 can detect full length (FL)PC1 and the C-terminal fragment (CTF).

From the laboratories of Feng Qian, PhD, and Terry J. Watnick, MD, University of Maryland, Baltimore
  1. Yu S, Hackmann K, Gao J, He X, Piontek K, García-González MA, Menezes LF, Xu H, Germino GG, Zuo J, Qian F. Essential role of cleavage of Polycystin-1 at G protein-coupled receptor proteolytic site for kidney tubular structure. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18688-93.

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