This mouse IgG1 monoclonal antibody [E4] was generated against 6xHis-tagged fusion protein and recognizes the C-lectin domain of human Polycystin-1 (PC1) (i.e. directed to N-terminal cleavage fragment).
Polycystin-1 (PC1) is the gene product of PKD1 that is mutated in human autosomal dominant polycystic Kidney disease. It is required for tubular morphogenesis and maintenance of the kidney and other organs. PC1 is a 11-transmembrane glycoprotein that is cic-autoproteolytically cleaved at juxtamembrane extracellular GPS/GAIN in vivo, resulting a N-terminal cleavage fragment and a C-terminal cleavage fragment in the kidney and other tissues.
From the laboratory of Feng Qian, PhD and Terry J. Watnick, MD, University of Maryland, Baltimore
|Reactivity:||Human and mouse|
|Immunogen:||6xHis-tagged fusion protein|
|Epitope:||C-lectin domain of human PC1|
|Purification Method:||Protein G Affinity|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB (1:200-500), IP(0.1-2ug per 2mg of protein), IF (1:50)|
Immunoprecipitation of endogenous polycystin-1 mouse lung tissue lysates E4 or 7E12. Immunoprecipitated PC1 is detected by immunoblot by 7E12 or anti-cc (directed against the c-terminal tail).
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