Anti-Complement C3b-iC3b [3E7] Antibody

This mouse IgG1 monoclonal antibody [3E7] was generated against human C3b and reacts with human and primate complement component 3b (C3b) and iC3b; blocks activation of the alternative pathway of complement.

Highlights

  • Reacts with both human and primate C3b and iC3b
  • Blocks activation of the alternative pathway of complement
  • Suitable for Western Blot, Immunohistochemistry and Neutralizing applications

The complement system consists of a number of small proteins found in the blood, generally synthesized by the liver, and normally circulating as inactive precursors (pro-proteins). When stimulated by one of several triggers, proteases in the system cleave specific proteins to release cytokines and initiate an amplifying cascade of further cleavages. The end-result of this activation cascade is massive amplification of the response and activation of the cell-killing membrane attack complex.

Complement component 3 (C3) plays a central role in the activation of the complement system. C3 cleavage by C3-convertase produces C3b and iC3b. C3b leads to an inflammatory response and also covalently binds to microbial cell surfaces aiding in opsonization of the microbe.

From the laboratory of Ronald P. Taylor, PhD, University of Virginia

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EG1006
Anti-Complement C3b-iC3b [3E7] Antibody
100ug In stock
Regular Price:$378.00
Specifications

Product Type: Antibody
Accession ID: NP_000055
Antigen: C3b
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 3 E7
Reactivity: Primate, Human
Immunogen: Human C3b
Species Immunized: Mouse
Purification Method: Protein G Affinity
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: IHC, WB, Neutralizing
Comments: 3E7 blocks activation of the alternative pathway of complement
Storage: -20C
Shipped: Cold packs

Provider
From the laboratory of Ronald P. Taylor, PhD, University of Virginia
References
  1. Lindorfer MA, Pawluczkowycz AW, Peek EM, Hickman K, Taylor RP, Parker CJ. A novel approach to preventing the hemolysis of paroxysmal nocturnal hemoglobinuria: both complement-mediated cytolysis and C3 deposition are blocked by a monoclonal antibody specific for the alternative pathway of complement. Blood. 2010 Mar 18;115(11):2283-91.
  2. DiLillo DJ, Pawluczkowycz AW, Peng W, Kennedy AD, Beum PV, Lindorfer MA, Taylor RP. Selective and efficient inhibition of the alternative pathway of complement by a mAb that recognizes C3b/iC3b. Mol Immunol. 2006 Mar;43(7):1010-9.
  3. Kennedy AD, Solga MD, Schuman TA, Chi AW, Lindorfer MA, Sutherland WM, Foley PL, Taylor RP. An anti-C3b(i) mAb enhances complement activation, C3b(i) deposition, and killing of CD20+ cells by rituximab. Blood. 2003 Feb 1;101(3):1071-9.

If you publish research with this product, please let us know so we can cite your paper.

Loading...