This mouse IgG2b monoclonal antibody [10C7] was generated against DNA immunization followed by recombinant protein and is specific for the N-terminal portion of Zaire ebolavirus polymerase cofactor VP35.
Ebola virus (EBOV), or sometimes referred to as Zaire ebolavirus is one of the five identified Ebola virus species. Zaire ebolavirus is a virological taxon that comes from the genus, Ebolvarius, family Filoviridae and the order Mononegavirales. Ebola, often fatal has been shown to cause hemorrhagic fever in humans and other mammals including monkeys and chimpanzees. Polymerase cofactor VP35 acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. It prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3). This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1. VP35 also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.
From the laboratory of Christopher F. Basler, PhD, Icahn School of Medicine at Mount Sinai
Part of The Investigator's Annexe program.
|Antigen:||Zaire ebolavirus polymerase cofacter VP35|
|Accession ID:||AF086833.2 Ebola Mayinga, Zaire 1976|
|Molecular Weight:||35 kDa|
|Immunogen:||DNA immunization followed by recombinant protein|
|Epitope:||Within the N-terminal portion of protein|
|Purification Method:||Protein G Affinity|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB (1:1000)|
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