Anti-Zaire Ebola Virus VP35 Protein, N-Terminal [10C7] Antibody

This mouse IgG2b monoclonal antibody [10C7] was generated against DNA immunization followed by recombinant protein and is specific for the N-terminal portion of Zaire ebolavirus polymerase cofactor VP35.

Highlights

  • Reacts with N-terminal portion of Zaire ebolavirus polymerase cofactor VP35
  • Suitable for Western Blot applications

Ebola virus (EBOV), or sometimes referred to as Zaire ebolavirus is one of the five identified Ebola virus species. Zaire ebolavirus is a virological taxon that comes from the genus, Ebolvarius, family Filoviridae and the order Mononegavirales. Ebola, often fatal has been shown to cause hemorrhagic fever in humans and other mammals including monkeys and chimpanzees. Polymerase cofactor VP35 acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. It prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3). This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1. VP35 also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.

From the laboratory of Christopher F. Basler, PhD, Icahn School of Medicine at Mount Sinai

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EMS703
Anti-Zaire Ebola Virus VP35 Protein, N-Terminal [10C7] Antibody
100ug In stock
Regular Price:$403.00
Specifications

Product Type: Antibody
Antigen: Zaire ebolavirus polymerase cofacter VP35
Accession ID: AF086833.2 Ebola Mayinga, Zaire 1976
Molecular Weight: 35 kDa
Isotype: IgG2b
Clonality: Monoclonal
Clone Name: 10C7
Reactivity: Virus
Immunogen: DNA immunization followed by recombinant protein
Species Immunized: Mouse
Epitope: Within the N-terminal portion of protein
Purification Method: Protein G Affinity
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1:1000)
Storage: -20C
Shipped: Cold packs

Provider
From the laboratory of Christopher F. Basler, PhD, Icahn School of Medicine at Mount Sinai
References
  1. Radwańska MJ, Jaskółowski M, Davydova E, Derewenda U, Miyake T, Engel DA, Kossiakoff AA, Derewenda ZS. The structure of the C-terminal domain of the nucleoprotein from the Bundibugyo strain of the Ebola virus in complex with a pan-specific synthetic Fab. Acta Crystallogr D Struct Biol. 2018 Jul 1;74(Pt 7):681-689. View Article
  2. Greenberg A, Huber BR, Liu DX, et al. Quantification of Viral and Host Biomarkers in the Liver of Rhesus Macaques: A Longitudinal Study of Zaire Ebolavirus Strain Kikwit (EBOV/Kik). Am J Pathol. 2020;190(7):1449-1460. View Article

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