Retinoblastoma, RB1 Negative Cell Line (RB143)
Retinoblastoma Cell Line (RB143) lacks expression of RB1 as a result of a single large deletion encompassing exons 3–17.
Retinoblastoma (RB), an intraocular tumor of childhood, is commonly associated with mutations in the RB1 gene. Loss of RB1 function is associated with a variety of human cancers, while inactivation of the RB1 tumor suppressor gene has been reported in several human malignancies in addition to RB, such as cancers of the breast, prostate, and lung. Furthermore, the RB1 gene family is intimately involved in the control of cellular proliferation, survival, and differentiation pathways in many mammalian cells.
Also available: Retinoblastoma Cell Line (RB116)
From the laboratory of Bruce R. Ksander, PhD, Schepens Eye Research Institute.
Part of The Investigator's Annexe program.
Retinoblastoma Cell Line (RB143) lacks expression of RB1 as a result of a single large deletion encompassing exons 3–17.
Retinoblastoma (RB), an intraocular tumor of childhood, is commonly associated with mutations in the RB1 gene. Loss of RB1 function is associated with a variety of human cancers, while inactivation of the RB1 tumor suppressor gene has been reported in several human malignancies in addition to RB, such as cancers of the breast, prostate, and lung. Furthermore, the RB1 gene family is intimately involved in the control of cellular proliferation, survival, and differentiation pathways in many mammalian cells.
Also available: Retinoblastoma Cell Line (RB116)
From the laboratory of Bruce R. Ksander, PhD, Schepens Eye Research Institute.
Part of The Investigator's Annexe program.
| Product Type: | Cell Line |
| Name: | RB143 |
| Cell Type: | Retinoblastoma |
| Accession ID: | CVCL_5I30 |
| Source: | Established from an explant of a large primary human RB tumor and provided for this study without patient identifiers |
| Organism: | Human |
| Morphology: | Multicellular clusters |
| Biosafety Level: | BSL1 |
| Growth Conditions: | 37C, 5% CO2; RPMI1640 medium , 10% FBS, penicillin (100 ?g/ml)-streptomycin (100 ?g/ml), 2 mM l-glutamine, 10 mM HEPES, 55 ?M 2-mercaptoethanol |
| Storage: | Liquid nitrogen |
| Shipped: | Dry ice |
RBI gene expression in RB116, RB143 and breast cancer MDA-MB231 cells
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis shows RB1 mRNA expression in RB116 cells. RB116 cells were evaluated alongside RB1-negative RB143 cells and RB1-positive MDA-MB231 breast cancer cells. RB116 cell expression was set at 1.0. The experiment was repeated three times and the error bars show standard deviation. No p values were calculated, as this was an experiment to determine presence or absence of RB1 and not meant to be comparative between cell lines. RB1 mRNA (mRNA) was detected in both RB116 and MDA-MB231 cells.
Adapted from: Bejjani A., et al. Mol Vis. 2012;18:2805-13. Epub 2012 Nov 29.
- Theodoropoulou S, Kolovou PE, Morizane Y, Kayama M, Nicolaou F, Miller JW, Gragoudas E, Ksander BR, Vavvas DG. Retinoblastoma cells are inhibited by aminoimidazole carboxamide ribonucleotide (AICAR) partially through activation of AMP-dependent kinase. FASEB J. 2010 Aug;24(8):2620-30. doi: 10.1096/fj.09-152546. Epub 2010 Apr 6.
- Bejjani A, Choi MR, Cassidy L, Collins DW, O'Brien JM, Murray T, Ksander BR, Seigel GM. RB116: an RB1+ retinoblastoma cell line expressing primitive markers. Mol Vis. 2012;18:2805-13. Epub 2012 Nov 29.
- Asnaghi L, White DT, Key N, Choi J, Mahale A, Alkatan H, Edward DP, Elkhamary SM, Al-Mesfer S, Maktabi A, Hurtado CG, Lee GY, Carcaboso AM, Mumm JS, Safieh LA, Eberhart CG. ACVR1C/SMAD2 signaling promotes invasion and growth in retinoblastoma. Oncogene. 2019 Mar;38(12):2056-2075. View Article
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