These mouse monoclonal antibodies were raised against bacterially expressed fragment (close to the Sun domain) of mouse SUN domain-containing protein 1 (Sun1) and recognize mouse SUN1.
SUN (Sad1, UNC-84) domain-containing proteins are type II membrane proteins that span the inner nuclear membrane. SUN proteins interact with lamins and other proteins in the nucleoplasm and with KASH (Klarsicht, ANC-1, and Syne homology) domain-containing proteins in the perinuclear space. Since the KASH proteins span the outer nuclear membrane and interact with components of the cytoskeleton, the SUN-KASH complex, also known as the LINC complex (LInker of the Nucleoskeleton and Cytoskeleton), provides a physical link between the nuclear interior and the cytoskeleton. The LINC complex is involved in diverse cellular functions including nuclear architecture, nuclear anchorage and migration, meiotic chromosome movement and cytoskeletal organization. Not surprisingly, aberrations in the LINC complex have been associated with number of human genetic diseases, including muscular dystrophy, cerebellar ataxia, hearing loss and infertility. Sun1 is one of at least five mammalian SUN protein family members and is ubiquitously expressed. Of note, Sun1 undergoes extensive alternative splicing. Human and mouse tissues express simultaneously several isoforms with the presence and prevalence of specific isoforms being tissue dependent. The BBmSun1 X15.15 antibody is predicted to detect all the known Sun1 isoforms.
From a laboratory at Agency for Science, Technology and Research (A*STAR).
|Accession ID:||O66837, 1193255|
|Molecular Weight:||102 kDa|
BBmSun1 X12.11: IgG2bk
BBmSun1 X15.15: IgG2ak
|Clone Name:||BBmSun1X12.11 or BBmSun1X15.15|
|Immunogen:||Bacterially expressed fragment (close to the Sun domain) of mouse Sun1|
|Purification Method:||Protein G|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry|
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