Arabinogalactan Protein (AGP) Antibodies

These rat IgM monoclonal antibodies were generated against polysaccharide and recognize land plants arabinogalactan protein (AGP).

Highlights:

  • Reacts with land plants AGP
  • Suitable for Immunofluorescence and ELISA (Clone LM2 only) applications

Generated to rice arabinogalactan-proteins (AGPs). Recognizes a carbohydrate epitope containing Β-linked glucuronic acid. It can recognize AGPs in several species. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-Ο-methyl-Β-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-Ο-methyl-Β-D-GlcA. Arabinogalactans are plant glycans composed primarily of arabinosyl and/or galactosyl residues. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.

From the laboratory of Paul Knox, PhD, University of Leeds.

Catalog Number Product Size AVAILABILITY Price Qty
ELD021
Anti-Arabinogalactan-protein (AGP) [LM2] Antibody
5mL (supernatant) In stock
Regular Price:$299.00
ELD023
Anti-Arabinogalactan-Protein (AGP) [JIM4] Antibody
5mL (supernatant) 3-5 weeks
Regular Price:$299.00
ELD024
Anti-Arabinogalactan-Protein (AGP) [JIM8] Antibody
5mL (supernatant) 3-5 weeks
Regular Price:$299.00
ELD025
Anti-Arabinogalactan-Protein (AGP) [JIM13] Antibody
5mL (supernatant) 3-5 weeks
Regular Price:$299.00
ELD026
Anti-Arabinogalactan-Protein (AGP) [LM14] Antibody
5mL (supernatant) 3-5 weeks
Regular Price:$299.00
ELD027
Anti-Arabinogalactan-Protein (AGP) [JIM15] Antibody
5mL (supernatant) 3-5 weeks
Regular Price:$299.00
ELD028
Anti-Arabinogalactan-Protein (AGP) [JIM16] Antibody
5mL (supernatant) 3-5 weeks
Regular Price:$299.00
ELD029
Anti-Arabinogalactan-Protein (AGP) [LM30] Antibody
5mL (supernatant) 3-5 weeks
Regular Price:$299.00
Specifications

Product Type: Antibody
Antigen: Arabinogalactan-protein (AGP)
Isotype: IgM (except JIM8, IgG2c)
Clonality: Monoclonal
Clone Name: Jim4, 8, 13, 15, 16 and LM2, 14, 30
Reactivity: Land Plants
Immunogen: Polysaccharide
Species Immunized: Rat
Epitope: LM2 and LM14: Bind to Glucuronosyl residue of AGP, LM30: Binds to Arabinosyl residues of AGP, JIM16: Binds to Galactosyl residue of AGP, Others: unknown
Buffer: Cell Culture Supernatant, 0.05% Sodium Azide
Tested Applications: IF 1:10, ELISA 1:10 (LM2 only)
Storage: -20C
Shipped: Room Temperature

Provider
From the laboratory of Paul Knox, PhD, University of Leeds.
References
  1. Smallwood M, Yates EA, Willats WGT, Martin H, Knox JP (1996) Immunochemical comparison of membrane-associated and secreted arabinogalactan-proteins in rice and carrot. Planta 198, 452-459
  2. Wilkinson et al. (2017) J. Cereal Science 74, 155-164.
  3. Stacey NJ, Roberts K, Knox JP. Patterns of expression of the JIM4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in Daucus carota L. Planta. 1990;180(2):285‐292.
  4. Yates EA, Valdor JF, Haslam SM, et al. Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology. 1996;6(2):131‐139.
  5. Pennell RI, Janniche L, Kjellbom P, Scofield GN, Peart JM, Roberts K. Developmental Regulation of a Plasma Membrane Arabinogalactan Protein Epitope in Oilseed Rape Flowers. Plant Cell. 1991;3(12):1317‐1326.
  6. Knox JP, Linstead PJ, Cooper JPC, Roberts K. Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J. 1991;1(3):317‐326.
  7. Moller I, Marcus SE, Haeger A, et al. High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles. Glycoconj J. 2008;25(1):37‐48.

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