These rat IgM monoclonal antibodies were generated against polysaccharide and recognize land plants arabinogalactan protein (AGP).
Generated to rice arabinogalactan-proteins (AGPs). Recognizes a carbohydrate epitope containing Β-linked glucuronic acid. It can recognize AGPs in several species. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-Ο-methyl-Β-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-Ο-methyl-Β-D-GlcA. Arabinogalactans are plant glycans composed primarily of arabinosyl and/or galactosyl residues. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
From the laboratory of Paul Knox, PhD, University of Leeds.
|Isotype:||IgM (except JIM8, IgG2c)|
|Clone Name:||Jim4, 8, 13, 15, 16 and LM2, 14, 30|
|Epitope:||LM2 and LM14: Bind to Glucuronosyl residue of AGP, LM30: Binds to Arabinosyl residues of AGP, JIM16: Binds to Galactosyl residue of AGP, Others: unknown|
|Buffer:||Cell Culture Supernatant, 0.05% Sodium Azide|
|Tested Applications:||IF 1:10, ELISA 1:10 (LM2 only)|
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