This reagent is a Cyanine7-conjugated, PEG-modified hexapeptide that specifically binds the formylpeptide receptor (FPR) of neutrophils. After tailvein injection of the agent, sites of inflammation, where neutrophils have been recruited and activated, can be imaged in vivo by near-infrared (NIR) fluorescence.
(Left) Representative fluorescence image at 3 hrs post injection of Neutrophil-Specific, NIR Fluorescent Imaging Agent (cFLFLF-PEG76-Cyanine7) (2 nmol): A, normal group; B, blocking group pre-injected with 100 nmol (50 eq.) of a non-fluorescence blocking peptide 1 hr before injection of the Neutrophil-Specific, NIR Fluorescent Imaging Agent.
From the laboratory of Dongfeng Pan, PhD, University of Virginia.
Read related blog post, Development of a Fluorescent Imaging Probe Specific for Neutrophils »
Part of The Investigator's Annexe program.
|Product Type:||Small Molecule|
|Name:||Neutrophil-Specific, NIR Fluorescent Imaging Agent (cFLFLF-PEG76-Cyanine7)|
|Molecular Weight:||5 kDa (average calculated mass) 0.25mg = 50nmol|
|Tested Applications:||in vivo imaging|
|Solubility:||Soluble in water and PBS. Create stock solution of 20uM by dissolving 0.25mg into 2.5ml of sterile water or PBS.|
|Ex/Em Maxima:||Ex: 745nm|
|Storage:||Protect from light. Once reconstituted, store aliquoted at -20C for up to 3 months (avoid repeated freeze / thaw cycles). For long term storage, keep lyophilized at -20C for up to 12 months.|
Dissolve Neutrophil-Specific, NIR Fluorescent Imaging Agent in water or neutral buffer for a working solution concentration of 20uM. Inject mice with 50uL Working Solution per 10g body weight via intravenous injection.
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