Mouse embryonic fibroblast (MEF) cell lines with floxed Actb (beta-actin), Actg1 (gamma-actin), or Actb/Actg1 alleles. Gene knock-out (KO) under exogenous expression of Cre recombinase via Cre-Lox recombination.
Beta-actin is a highly conserved protein involved in cell motility, structure, and integrity. Beta-actin, is one of the six isoforms in humans, and one of the two non-muscle cytoskeletal actins. Similar to beta-actin, gamma actin is widely expressed in cellular cytoskeletons of many tissues; in adult striated muscle cells, gamma-actin is localized to Z-discs and costamere structures, which are responsible for force transduction and transmission in muscle cells.
From the laboratory of James M. Ervasti, PhD, University Minnesota, Twin Cities.
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|Product Type:||Cell Line|
|Cell Type:||Embryonic fibroblast|
|Source:||Day 13.5 mouse embryos|
|Subculturing:||Fast growing immortalized mouse embryonic fibroblasts, 1:10 Split|
|Growth Conditions:||DMEM, 10% FBS, 1% Pen Strep, 0.5ug/mL Fungizone|
|Cryopreservation:||Media + 5% DMSO|
|Comments:||Cre-Lox recombination gene knock-out requires exogenous Cre expression. See: Patrinostro X, et al. Mol Biol Cell. 2017 Mar 15;28(6):771-782.|
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