These IgG2, k light chain mouse monoclonal antibodies were raised against protein and recognize zebrafish novel immune-type receptor 9 (Nitr9).
The novel immune-type receptor (NITR) family includes activating and inhibitory forms that are predicted to function as natural killer receptors in teleost species. The only activating NITR gene in zebrafish is nitr9. The zebrafish nitr9 gene yields three alternatively spliced transcripts that encode three proteins isoforms: Nitr9-long (Nitr9L), Nitr9-short (Nitr9S), and Nitr9-supershort (Nitr9SS). Nitr9 transcripts are detectable in immune tissues, but are present at significantly higher levels in lymphocytes than in myeloid cells. The Nitr9L protein has been shown to physically partner with and signals via the adaptor protein Dap12.
From the laboratory of Jeffrey A. Yoder, PhD, North Carolina State University.
|Antigen:||Zebrafish novel immune-type receptor 9 (Nitr9)|
|Accession ID:||GenBank AY570237.1, AY570238.1, EF690658.1|
|Molecular Weight:||34 kDa (Nitr9L), 30 kDa (Nitr9S) and 20 kDa (Nitr9SS). Nitr9L and Nitr9S may appear larger in western analyses due to glycosylation|
19.1.1: IgG2a, k light chain
90.10.5: IgG2b, k light chain
|Epitope:||I-type Immunoglobulin domain (encoded by nucleotides 298-623 of GenBank NM 001005576.1)|
|Purification Method:||Protein G|
|Buffer:||PBS, 0.05% (w/v) Sodium Azide|
19.1.1: IF and FC with transfected cells (may not recognize Nitr9S isoform by FC)
90.10.5: WB with endogenous tissue (1:1000), IF and FC with transfected cells (may not recognize Nitr9S isoform by FC)
|Comments:||May not recognize Nitr9S isoform by FC|
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