Erythromycin A Production Plasmids
Plasmids for the expression of erythromycin A using E coli.
Erythromycin A is a potent antibiotic long-recognized as a therapeutic option for bacterial infections. The soil-dwelling bacterium Saccharopolyspora erythraea natively produces erythromycin A from a 55 kb gene cluster composed of three large polyketide synthase genes (each ~10 kb) and 17 additional genes responsible for deoxysugar biosynthesis, macrolide tailoring, and resistance.
From the laboratory of Blaine A. Pfeifer, PhD, Tufts University.
Plasmids for the expression of erythromycin A using E coli.
Erythromycin A is a potent antibiotic long-recognized as a therapeutic option for bacterial infections. The soil-dwelling bacterium Saccharopolyspora erythraea natively produces erythromycin A from a 55 kb gene cluster composed of three large polyketide synthase genes (each ~10 kb) and 17 additional genes responsible for deoxysugar biosynthesis, macrolide tailoring, and resistance.
From the laboratory of Blaine A. Pfeifer, PhD, Tufts University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Plasmid |
| Gene/insert name: | pHZT1, pHZT2 |
| Antibiotic Resistance: |
pHZT1: Kan pHZT2: Carb |
| Fusion Tag(s): |
pHZT1: eryBI,BII,BIII,BIV,BV,BVI,BVII,ermE pHZT2: eryCI,CII,CIII,CIV,CV,CVI,eryF,eryG,eryK |
| Grow in E. coli at 37 C: | Yes |
| Cloning Site 5': | XbaI |
| Cloning Site 3': | SpeI |
| Insert Size: | 13.2 kb |
| Vector Backbone and Size: |
pHZT1: pET28a, 5369 bp pHZT2: pET21c, 5441 bp |
| High or low copy: | Low |
| Storage: | -20C |
| Shipped: | Room Temperature |
Provider
From the laboratory of Blaine A. Pfeifer, PhD, Tufts University.
Comments
Adapted from: Zhang, Haoran, et al. Chemistry & biology 17.11 (2010): 1232-1240.
References
- Zhang, Haoran, et al. "Complete biosynthesis of erythromycin A and designed analogs using E. coli as a heterologous host." Chemistry & biology 17.11 (2010): 1232-1240.
If you publish research with this product, please let us know so we can cite your paper.
