Anti-Centromere Protein F (CENP-F) Antibodies
These IgG mouse monoclonal antibodies were produced against mouse c-terminal centromere binding protein F (CENP-F) and recognize human, mouse, dog and quail CENP-F.
Highlights:
- Reacts with human, mouse, dog and quail CENP-F in cell lines derived from these organisms
- Characterization studies show that all antibodies react with transfected CENP-F expressed in COS cells in Western Blot and immunoflourescence assays.
- Antibodies react with endogenous CENP-F using immunoflourescence assays using cultured cells derived from the species listed above
- Suitable for Immunofluorescence applications
C-terminal domain of centromere binding protein F associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. Shown to be involved in the regulation of DNA synthesis, cell cycle progression. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. Examination of the literature on CENP-F reveals great complexity in function, expression, and cellular localization in different cell types and different species.
From the laboratory of David M. Bader, PhD, Vanderbilt University.
These IgG mouse monoclonal antibodies were produced against mouse c-terminal centromere binding protein F (CENP-F) and recognize human, mouse, dog and quail CENP-F.
Highlights:
- Reacts with human, mouse, dog and quail CENP-F in cell lines derived from these organisms
- Characterization studies show that all antibodies react with transfected CENP-F expressed in COS cells in Western Blot and immunoflourescence assays.
- Antibodies react with endogenous CENP-F using immunoflourescence assays using cultured cells derived from the species listed above
- Suitable for Immunofluorescence applications
C-terminal domain of centromere binding protein F associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. Shown to be involved in the regulation of DNA synthesis, cell cycle progression. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. Examination of the literature on CENP-F reveals great complexity in function, expression, and cellular localization in different cell types and different species.
From the laboratory of David M. Bader, PhD, Vanderbilt University.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Centromere Binding Protein F (CENP-F) |
| Accession ID: | P49454 |
| Molecular Weight: | 347-380 kDa |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| Clone Name: |
REMELDA 9 REMELDA 16 REMELDA 17 REMELDA 18 REMELDA 30 |
| Reactivity: | Human, mouse, dog and quail |
| Immunogen: | Mouse C-terminal CENP-F |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | IF |
| Comments: | This monoclonal antibody works at high specificity with little background when used in hybridoma medium. Purification is not needed for standard immunochemical analyses. |
| Storage: | -80C |
| Shipped: | Cold Packs |
Note: Variation in subcellular protein localization may be observed using diverse cell types from different organisms.
| Clone | Hela | HCT | MDCK | QM7 |
| Remelda 9 | Mitotic Spindle | Mitotic Spindle | - | Mitotic Spindle |
| Remelda 16 | Peripheral cytoplasmic domain | Broad cytoplasm | Perinuclear/small nuclear puncta | Perinuclear/small nuclear puncta |
| Remelda 17 | Peripheral cytoplasmic domain/ Large nuclear puncta | Large nuclear puncta | Cytoplasmic strands | Small nuclear puncta/peripheral cytoplasmic domain |
| Remelda 18 | Perinuclear | Perinuclear | Small nuclear puncta | Small nuclear puncta |
| Remelda 30 | Large nuclear puncta | Large nuclear puncta | Large nuclear puncta | Cytoplasm |
- Reid W. McCullough, Emily L. Mace, Elise R. Pfaltzgraff, and David M. Bader. Monoclonal Antibodies against Centromere Binding Protein F. In Review.
If you publish research with this product, please let us know so we can cite your paper.
