Monosomy 3 Uveal Melanoma Cell Lines
Cell lines UPMM-1 and UPMM-2 are derived from different donors with primary uveal melanoma tumors with monosomy 3.
Highlights:
- Cells are positive for melanoma markers HMB45, S100 and MCSP
- UPMM-1 -Monosomy 3; GNAQ, R183 mutation (retains the chromosome 3 status of the primary tumor)
- UPMM-2 -Monosomy 3; GNAQ, Q209 mutation (chromosome 3 has undergone duplication (isodisomy) and is present on the background of a hypotetraploid karyotype)
Uveal melanoma (UM) is a cancer (melanoma) of the eye involving the iris, ciliary body, or choroid (collectively referred to as the uvea). Tumors arise from the pigment cells (melanocytes) that reside within the uvea giving color to the eye. Primary UM can be devided in two major classes. Metastatic progression is typically
seen in one class of UM characterized by monosomy 3, but is rare in tumors with disomy
3. Therefore, monosomy 3 cell lines are most suitable for analyses of mechanisms and
pathways contributing to metastatic progression. There is a tremendous market need,
because currently only one alternative cell line is available with monosomy 3.
From the laboratory of Michael Zeschnigk, PhD, University of Duisburg-Essen.
Cell lines UPMM-1 and UPMM-2 are derived from different donors with primary uveal melanoma tumors with monosomy 3.
Highlights:
- Cells are positive for melanoma markers HMB45, S100 and MCSP
- UPMM-1 -Monosomy 3; GNAQ, R183 mutation (retains the chromosome 3 status of the primary tumor)
- UPMM-2 -Monosomy 3; GNAQ, Q209 mutation (chromosome 3 has undergone duplication (isodisomy) and is present on the background of a hypotetraploid karyotype)
Uveal melanoma (UM) is a cancer (melanoma) of the eye involving the iris, ciliary body, or choroid (collectively referred to as the uvea). Tumors arise from the pigment cells (melanocytes) that reside within the uvea giving color to the eye. Primary UM can be devided in two major classes. Metastatic progression is typically seen in one class of UM characterized by monosomy 3, but is rare in tumors with disomy 3. Therefore, monosomy 3 cell lines are most suitable for analyses of mechanisms and pathways contributing to metastatic progression. There is a tremendous market need, because currently only one alternative cell line is available with monosomy 3.
From the laboratory of Michael Zeschnigk, PhD, University of Duisburg-Essen.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Cell Line |
| Accession ID: | CVCL_C299 |
| Organism: | Human |
| Source: | Uveal Melanoma |
| Subculturing: | Split 1x a week |
| Growth Conditions: | Ham's F12 with 10-20% FCS, 100 IU/ml penicillin, 100ug/ml streptomycin, CO2 5%, O2 20% |
| Donor Attributes: | UPMM-1 amd UPMM-2 are from different donors (both are from primary tumors with monosomy 3 in male caucasian subjects) |
| Comments: | Karyotyping performed via conventional GTG-banding. See: Nareyeck G, et al. Exp Eye Res. 2006 Oct;83(4):858-64. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
- Nareyeck G, Zeschnigk M, Prescher G, Lohmann DR, Anastassiou G. Establishment and characterization of two uveal melanoma cell lines derived from tumors with loss of one chromosome 3. Exp Eye Res. 2006 Oct;83(4):858-64. Epub 2006 Jun 5. Review. PubMed PMID: 16750193.
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