These rabbit polyclonal antibodies were generated against BSA conjugated ecdysteroids E and 20E and, in combination with BLG-conjugated ecdysteroids E and 20E, allow for a fast, accurate and low-cost means to measure titers for ecdysone (E) and 20-hydroxyecdysone (20E).
Ecdysteroid hormones regulate several aspects of insect development and reproduction. The predominant ecdysteroids produced by insects including mosquitoes are ecdysone (E) and 20-hydroxyecdysone (20E). The ability to measure E and 20E titers is essential for many studies. These antibodies BLG-conjugated antigens allow for a fast, accurate and low-cost means to measures titers for ecdysone (E) and 20-hydroxyecdysone (20E).
EAB25: specific for 20-Hydroxyecdysone (20E)
EAB27: recognizes both ecdysone (E) and 20-hydroxyecdysone (20E), and somewhat makisterone A
|Buffer:||Whole serum for antibodies, PBS with 0.02% sodium azide for Ecdysteroid-BLG conjugates|
|Tested Applications:||Enzymelinked immunoassay (EIA) (Protocol); Not suitable for immunocytochemistry or western blots|
|Comments:||Antisera EAB25 and EAB27 were produced to ecdysone (E) conjugated to bovine serum albumin (BSA) or to 20-Hydroxyecdysone (20E) conjugated to BSA, so they should not be used in an enzyme immunoassay with BSA.|
Table of Antisera Properties
|Antisera (antigen)||Affinity||Final use conc.||Detection Range (pg)|
|EAB25 (20E-BSA)||20E||1:40,000||20E: 30-2,000|
|EAB27 (E-BSA)||E, 20E||1:3,000||E/20E: 4-500|
Adapted from: McKinney DA, et al. (2017) Gen Comp Endocrinol. 253:60-69.
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