Lambda-LIZ transgene harboring transformed MEF cell lines. Wild-type or null for: N-methylpurine DNA glycosylase (AAG), Mismatch repair endonuclease PMS2 (PMS2), DNA polymerase beta (Beta-pol), or Uracil-DNA glycosylase (UNG).
Of great utility in toxicology and DNA repair research are cell lines derived from gene knockout mice enabling one to evaluate the formation and accumulation of gene mutations as a direct function of base excision repair (AAG, Beta-pol, UNG) and/or mismatch repair (PMS2). Of particular importance are lambda-LIZ transgenes. The utility of these fibroblast cell lines (derived embryos) stem from the deficiency in base excision repair, as a result of the null mutation in the AAG, Beta-pol, or UNG genes or a deficiency in mismatch repair, as a result of the null mutation in the PMS2 gene. In addition, in some cases, the cells have a double KO: AAG/Beta-pol or PMS2/Beta-pol.
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|Product Type:||Cell Line|
|Name:||DNA Damage/Repair Transformed MEF Cell Lines (92TAg, 88TAg, 308TAg, 127TAg, 151TAg, 283TAg, 207TAg, 210TAg)|
|Cell Type:||Transformed MEF|
|Growth Conditions:||DMEM high glucose (Invitrogen cat# 11960-044), FBS 10% (50ml), Pen/strep (5.5ml)(Invitrogen cat# 15070-063), Glutamx (11ml) (Invitrogen cat# 35050-061); Cells should be grown at 10% CO2|
|Cryopreservation:||One million cells/ml and stored in 70% Media A/20% FBS/10% DMSO; one ml per vial.|
|Mycoplasma Tested:||August 2016 - Negative|
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