Anti-Actin-7 Antibodies

These mouse monoclonal antibodies were generated against Arabidopsis thaliana Actin-7 and recognize A. thaliana Actin-7.

Highlights:

  • React with A. thaliana Actin-7 (May display reactivity toward multiple other especies)
  • Suitable for Western Blot, ELISA and Immunofluorescence applications

Actin-7 has been identified in animals, plants and protists. During plant growth and development, the phytohormone auxin induces a wide array of changes that include cell division, cell expansion, cell differentiation, and organ initiation. It has been suggested that the actin cytoskeleton plays an active role in the elaboration of these responses by directing specific changes in cell morphology and cytoarchitecture. The promoter and protein product of ACT7, an Arabdopsis vegetative actin gene, are rapidly and strongly induced in response to exogenous auxin. ACT 7 is required for the formation of callus tissue, and is also essential for germination and root growth ACT7 actin gene is also expressed in rapidly developing tissues and responds to external stimuli, such as exposure to hormones.

From the laboratory of Richard B. Meagher, PhD, University of Georgia.

Catalog Number Product Size AVAILABILITY Price Qty
EGA245
Anti-Actin-7 [29G12] Antibody, 100ug
100ug 2-3 weks
Regular Price:$306.00
EGA246
Anti-Actin-7 [33E8] Antibody, 100ug
100ug 2-3 weks
Regular Price:$306.00
EGA247
Anti-Actin-7 [36H8] Antibody, 100ug
100ug 2-3 weks
Regular Price:$306.00
Specifications

Product Type: Antibody
Name: Actin-7 Antibodies
Antigen: Actin-7
Accession ID: P53492
Isotype: IgG
Clonality: Monoclonal
Clone Name: 29G12.G5.G6, 33E8.C11.F5.D1, 36H8.C12.H10.B6
Reactivity: Tested in A. thaliana. May display reactivity toward multiple other especies (ND)
Immunogen: A. thaliana Actin-7
Species Immunized: BALB/c mouse
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB, ELISA, IF (It is advisable to use all three MAbs in the first-time, qualitative experimental setup to determine which is the most suitable for a specific experiment. These may be used yo complement excperiments carried out involving mAbGEa universal anti-actins antibody.)
Storage: -20C
Shipped: Cold packs

Provider
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
Comments
It is advisable to use all three MAbs in the first-time, qualitative experimental setup to determine which is the most suitable for a specific experiment. These may be used yo complement excperiments carried out involving mAbGEa universal anti-actins antibody.
References
  1. Kandasamy, M.K., Gilliland, L.U., McKinney, E.C., and Meagher, R.B. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell 13, 1541-54.
  2. Kandasamy, M.K., McKinney, E.C., and Meagher, R.B. (1999). The late pollen-specific actins in angiosperms. Plant J 18, 681-91.
  3. Kandasamy, M.K., McKinney, E.C., Roy, E., and Meagher, R.B. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell 24, 2041-57.
  4. L. U. Gilliland, L. C. Pawloski, M. K. Kandasamy, and R. B. Meagher, “Arabidopsis actin gene ACT7 plays an essential role in germination and root growth,” Plant Journal, vol. 33, no. 2, pp. 319–328, 2003.
  5. McDowell, JM, An, Y-q, Huang,S, McKinney, EC, Meagher RB: The Arabidopsis ACT7Actin Gene Is Expressed in Rapidly Developing Tissues and Responds to Several External Stimuli. Plant Physiol. (1 996) 111 : 699-711.

If you publish research with this product, please let us know so we can cite your paper.

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