These mouse monoclonal antibodies were generated against Arabidopsis thaliana Actin-7 and recognize A. thaliana Actin-7.
Actin-7 has been identified in animals, plants and protists. During plant growth and development, the phytohormone auxin induces a wide array of changes that include cell division, cell expansion, cell differentiation, and organ initiation. It has been suggested that the actin cytoskeleton plays an active role in the elaboration of these responses by directing specific changes in cell morphology and cytoarchitecture. The promoter and protein product of ACT7, an Arabdopsis vegetative actin gene, are rapidly and strongly induced in response to exogenous auxin. ACT 7 is required for the formation of callus tissue, and is also essential for germination and root growth ACT7 actin gene is also expressed in rapidly developing tissues and responds to external stimuli, such as exposure to hormones.
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
|Clone Name:||29G12.G5.G6, 33E8.C11.F5.D1, 36H8.C12.H10.B6|
|Reactivity:||Tested in A. thaliana. May display reactivity toward multiple other especies (ND)|
|Immunogen:||A. thaliana Actin-7|
|Species Immunized:||BALB/c mouse|
|Purification Method:||Protein G|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, ELISA, IF (It is advisable to use all three MAbs in the first-time, qualitative experimental setup to determine which is the most suitable for a specific experiment. These may be used yo complement excperiments carried out involving mAbGEa universal anti-actins antibody.)|
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