Residue-Free 3D Printed Cell Culture Inserts

These inserts are useful for seeding cells within custom and defined residue-free surface areas, which maximize cell seeding area and facilitate longer incubation times, promoting maximal cell attachment and cell spreading.


  • Allow accurate and reproducible cell seeding within custom-defined substrate areas in a wide array of cell culture uses
  • Deter solution evaporation which facilitates longer incubation times and promotes cell attachment and spreading
  • Emulate the normal physiological setting of the cells at experiment onset
  • Ideal for parallel plate flow chamber experiments where cell perfusion area is limited to the manufacturer’s defined gasket area

From the laboratories of David A. Tulis, PhD, FAHA and William E. Howard, PhD, East Carolina University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Residue-Free 3D Printed Cell Culture Inserts, 10/pk
10/pk In stock
Regular Price:$30.00
On Sale:
Residue-Free 3D Printed Cell Culture Inserts, 50/pk
50/pk In stock
Regular Price:$135.00
On Sale:

Dimensions: 2.3cm long, 1.3cm wide with a 0.5cm depth
Product Type: Kit
Material: Plastic (proprietary mixture of methacrylic acid esters and a photoinitiator)
Tested Applications: Parallel plate flow chamber experiments
Sterility: Non-sterile; To sterilize, hydrogen peroxide (3% solution) and isopropyl alcohol is recommended.
Number of wells: 1
Cell Seeding Area: 1.71cm2
Storage: Room temperature
Shipped: Ambient temperature

From the laboratories of David A. Tulis, PhD, FAHA and William E. Howard, PhD, East Carolina University.

Suggested Protocol

  1. Place 3D insert on flat surface.
  2. Seed cells at desired confluence using 500 µL of cell suspension and allow to adhere overnight.
  3. Carefully remove insert with forceps.
  4. Wash excess cells and residual conditioned media using a buffered saline solution.
  5. Add desired volume of growth media.
  1. Holt AW, Howard WE, Ables ET, George SM, Kukoly CA, Rabidou JE, Francisco JT, Chukwu AN, Tulis DA. Making the cut: Innovative methods for optimizing perfusion-based migration assays. Cytometry A. 2017 Mar;91(3):270-280. View Article

If you publish research with this product, please let us know so we can cite your paper.