Immortalized Drosophila adult muscle progenitor-like cells that can be differentiated in vitro upon ecdysone treatment.
These cell lines were derived from Drosophila embryonic primary cultures established from Act5C > UAS-RasV12, UAS-GFP embryos in which ubiquitously-expressed Gal4 drives the expression of both RasV12 and GFP (as described in Simcox et al., 2008). Although the cultures were prepared from whole embryos, the cell lines present many characteristics of Adult Muscle Progenitor cells (often compared to vertebrate muscle stem cells, or satellite cells). First, they express a number of genes usually expressed in AMPs (e.g., twist). Second, upon addition of the steroid hormone ecdysone, the cells can be differentiated into muscle cells, expressing terminal differentiation markers (e.g., myosin heavy chain) as described in Dequeant et al., 2015 with the R1 cell line being the most efficiently differentiated cell line into muscle cells.
From the laboratories of Norbert Perrimon, PhD, Harvard University and Amanda Simcox, PhD, The Ohio State University.
|Product Type:||Cell Line|
|Name:||R1 or Ras[V12]-H1R3 or Ras[V12]-H3R4 or Ras[V12]-H4R7 or Ras[V12]-H7|
|Cell Type:||Adult muscle progenitor-like cells|
|Accession ID:||R1, CVCL_5I26; R3, CVCL_1B60; R4, CVCL_5I27; R7, CVCL_1B61|
|Source:||Drosophila melanogasterembryonic primary culture|
|Subculturing:||Cells can be split 1:3, 1:5, or 1:10 depending on how often cells are needed for experiments. Trypsin-EDTA solution (1×) (Life Technologies).|
|Growth Conditions:||Schneiders insect medium (Gibco/Invitrogen, Cat. 21720-024) (100mL) supplemented with 11.25mL heat-inactivated fetal bovine serum (Gibco/Invitrogen, Cat. 16140-071) and 1.25mL antibiotic mix (Penicillin-Streptomycin liquid, Gibco/Invitrogen, Cat.15070-063). Grow at 19C.|
|Cryopreservation:||50% Schneiders medium, 30% serum and 20% dimethyl sulfoxide (DMSO)|
|Comments:||The cell lines also express GFP. The cell lines can be differentiated into muscle cells by addition of ecdysone as described in Dequeant et al., 2015.|
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