DYn-2 is a chemoselective probe for detecting sulfenylated proteins in intact cells.
Mild oxidation can convert the sulfhydryl group of cysteine residues on proteins to cysteine-sulfenic acid derivatives (Cys-SOH). Protein sulfenylation is a post-translational modification that is relevant to redox signaling. DYn-2 and DYn-2-d6 differ in molecular weight by 6 Da. and can be used for quantitative analysis of cells stimulated with reactive oxygen species.
From the laboratory of Kate Carroll, PhD, The Scripps Research Institute.
|Product Type:||Small Molecule|
|Solubility:||Stock solution in DMSO (up to 500 mM), then dilute to appropriate concentration in aqueous buffer with final DMSO concentration <5%|
|Stability:||>6 months at -30C|
|Spectral Information:||1H-NMR (400 MHz, CDCl3): δ 5.42 (s, 1H), 3.41 (d, J= 4.0 Hz, 2H), 2.75?1.72 (m, 16H), 1.68?1.45 (m, 6H). 13C-NMR (100 MHz, CDCl3): δ 204.9, 204.4, 197.0, 189.2, 104.2, 84.4, 84.1, 69.1, 68.9, 58.5, 49.1, 41.8, 39.9, 30.1, 29.6, 28.5, 26.4, 26.2, 26.1, 24.7, 18.8, 18.7. ESI-MS, m/z for C11H14O2: calculated, 178.23; observed, 179.1 [M + H+]|
|Storage:||Room temperature for short-term, -30 oC for long term storage.|
If you publish research with this product, please let us know so we can cite your paper.