This lentivirus expresses ASCL1 under the control of the Tet promoter.
ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1) is a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.
|Vector Information:||Vector includes both 5? and 3? lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)|
|Target Gene / Reporter(s):||Ascl1|
|Titer:||1 x 10^8TU/mL|
|Storage:||Store at -80C. Multiple freeze/thaw cycles not recommended.|
NOTE: This Inducible Tet-On lentivirus does not contain the reverse tetracycline-controlled transactivator (rtTA) protein, which is required for the Tet-On system. Unless the cells used stably produce rtTA, it is necessary to cotransduce this lentivirus with a rtTA lentivirus (rtTA (CMV, neomycin) Lentivirus or rtTA (CMV, puromycin) Lentivirus.
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