These mouse IgG monoclonal antibodies were generated against affinity-purified non-structural protein (NS1) and recognize a broad range of influenza virus NS1 proteins.
Influenza A viruses (IAV) are negative-sense, single-stranded, segmented RNA viruses. The NS1 influenza protein (NS1) is a viral nonstructural protein encoded by the NS gene segments of type A, B and C influenza viruses. NS1 inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. NS1 prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7.
From the laboratory of Kevin M. Coombs, PhD, University of Manitoba.
|Antigen:||Influenza virus NS1 protein|
|Molecular Weight:||26 kDa|
|Reactivity:||Broadly cross-reactive & highly NS1 specific. Reactive against multiple H1N1 and H3N2 subtypes, and, based on epitope mapping and sequence comparisons, predicted to react with > 90% of currently known H1-H16 NS1 types.|
|Immunogen:||Affinity-purified His6-tagged NS1 from A/Hong Kong/1/1968 (H3N2)|
|Purification Method:||Protein G|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||Western Blot, Immunofluorescence Microscopy, and Immunoprecipitation applications (other applications not yet tested)|
|5D6||Strong Reactivity||Weak Reactivity||12-DCFLWHVR-19|
|10C7||Strong Reactivity||Strong Reactivity||12-DCFLWHVR-19|
|7D11||Strong Reactivity||Medium Reactivity||29-DAPFLDR-35|
|5B10||Weak Reactivity||Medium Reactivity||138-FDRLETLILL-147|
|3F5||Strong Reactivity||Strong Reactivity||161-PLPSLPGH-169|
|5F4||Medium Reactivity||Strong Reactivity||161-PLPSLPGH-169|
|13D8||Strong Reactivity||Strong Reactivity||161-PLPSLPGH-169|
|8C7||Medium Reactivity||Strong Reactivity||161-PLPSLPGH-169|
|4E10||Medium Reactivity||No Reactivity||211-RPPLT-215|
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