Anti-Influenza A, Non-Structural Protein 1 (NS1) Antibodies

These mouse IgG monoclonal antibodies were generated against affinity-purified non-structural protein (NS1) and recognize a broad range of influenza virus NS1 proteins.

Highlights:

  • Broadly cross reactive & highly NS1 specific - Shown to recognize multiple H1N1 and H3N2 subtypes
  • Predicted to react with >90% of currently known H1-H16 NS1 types, based on epitope mapping and sequence comparisons
  • Potential diagnostic tool for tracking productive IAV replicates & characterizing NS1 structure & function
  • Nuclear & cytoplasmic detection as early as 5 hrs post-infection
  • Suitable for Western Blot, Immunofluorescence Microscopy, and Immunoprecipitation applications (other applications not yet tested)

Influenza A viruses (IAV) are negative-sense, single-stranded, segmented RNA viruses. The NS1 influenza protein (NS1) is a viral nonstructural protein encoded by the NS gene segments of type A, B and C influenza viruses. NS1 inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. NS1 prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7.

From the laboratory of Kevin M. Coombs, PhD, University of Manitoba.

Catalog Number Product Size AVAILABILITY Price Qty
EMB001
Anti-Influenza A, Non-Structural Protein 1 (NS1) [5D6] Antibody, 100ug
100ug 3-5 weeks
Regular Price:$306.00
EMB002
Anti-Influenza A, Non-Structural Protein 1 (NS1) [10C7] Antibody, 100ug
100ug In stock
Regular Price:$306.00
EMB003
Anti-Influenza A, Non-Structural Protein 1 (NS1) [7D11] Antibody, 100ug
100ug 3-5 weeks
Regular Price:$306.00
EMB004
Anti-Influenza A, Non-Structural Protein 1 (NS1) [5B10] Antibody, 100ug
100ug 3-5 weeks
Regular Price:$306.00
EMB005
Anti-Influenza A, Non-Structural Protein 1 (NS1) [3F5] Antibody, 100ug
100ug In stock
Regular Price:$306.00
EMB006
Anti-Influenza A, Non-Structural Protein 1 (NS1) [5F4] Antibody, 100ug
100ug 3-5 weeks
Regular Price:$306.00
EMB007
Anti-Influenza A, Non-Structural Protein 1 (NS1) [13D8] Antibody, 100ug
100ug 3-5 weeks
Regular Price:$306.00
EMB008
Anti-Influenza A, Non-Structural Protein 1 (NS1) [8C7] Antibody, 100ug
100ug In stock
Regular Price:$306.00
EMB009
Anti-Influenza A, Non-Structural Protein 1 (NS1) [4E10] Antibody, 100ug
100ug 3-5 weeks
Regular Price:$306.00
Specifications

Product Type: Antibody
Antigen: Influenza virus NS1 protein
Molecular Weight: 26 kDa
Isotype: IgG2a ?
Clone Name: 5D6
Reactivity: Broadly cross-reactive & highly NS1 specific. Reactive against multiple H1N1 and H3N2 subtypes, and, based on epitope mapping and sequence comparisons, predicted to react with > 90% of currently known H1-H16 NS1 types.
Immunogen: Affinity-purified His6-tagged NS1 from A/Hong Kong/1/1968 (H3N2)
Species Immunized: Mouse
Epitope: See Table
Purification Method: Protein G
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: Western Blot, Immunofluorescence Microscopy, and Immunoprecipitation applications (other applications not yet tested)
Storage: -20C
Shipped: Cold packs

Research
Clone Western Blot Immunofluorescence Epitope
5D6 Strong Reactivity Weak Reactivity 12-DCFLWHVR-19
10C7 Strong Reactivity Strong Reactivity 12-DCFLWHVR-19
7D11 Strong Reactivity Medium Reactivity 29-DAPFLDR-35
5B10 Weak Reactivity Medium Reactivity 138-FDRLETLILL-147
3F5 Strong Reactivity Strong Reactivity 161-PLPSLPGH-169
5F4 Medium Reactivity Strong Reactivity 161-PLPSLPGH-169
13D8 Strong Reactivity Strong Reactivity 161-PLPSLPGH-169
8C7 Medium Reactivity Strong Reactivity 161-PLPSLPGH-169
4E10 Medium Reactivity No Reactivity 211-RPPLT-215
Provider
From the laboratory of Kevin M. Coombs, PhD, University of Manitoba.
References
  1. Rahim MN, Selman M, Sauder PJ, Forbes NE, Stecho W, Xu W, Lebar M, Brown EG, Coombs KM. Generation and characterization of a new panel of broadly reactive anti-NS1 mAbs for detection of influenza A virus. J Gen Virol. 2013 Mar;94(Pt3):593-605
  2. Chaimayo C, Dunagan M, Hayashi T, Santoso N, Takimoto T. Specificity and functional interplay between influenza virus PA-X and NS1 shutoff activity. PLoS Pathog. 2018 Nov 29. View Article
  3. If you publish research with this product, please let us know so we can cite your paper.

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