Anti-Carbonic Anhydrase Antibodies

These rabbit IgG polyclonal antibodies recognize human, mouse, or rat carbonic anhydrases.

Highlights:

  • Recognize human, mouse, or rat carbonic anhydrases I, II, 4, 5a, 5b, 12 or 14
  • Suitable for Western Blot, Immunoprecipitation and Immunohistochemistry applications

The carbonic anhydrases, also referred as carbonate dehydratases, form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons that occurs in the absence of a catalyst. Carbonic anhydrases have been found to be abundant in all mammalian tissues, plants, algae and bacteria. There are three main classes of carbonic anhydrases, alpha known for mammals, beta for plant enzymes and gamma for methane producing bacteria. Members of these different classes share very little sequence or structural similarity, but they have the same function and require a zinc ion at the active site, therefore they are all classified as metalloenzymes.

From the laboratory of Abdul Waheed, PhD, St. Louis University.

Catalog Number Product Size AVAILABILITY Price Qty
ESL202
Anti-Carbonic Anhydrase 12 (human) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL204
Anti-Carbonic Anhydrase 4 (human) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL205
Anti-Carbonic Anhydrase 12 (mouse) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL206
Anti-Carbonic Anhydrase 14 (mouse) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL207
Anti-Carbonic Anhydrase 5A (mouse) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL208
Anti-Carbonic Anhydrase 5B (mouse) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL209
Anti-Carbonic Anhydrase 5A (human) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL210
Anti-Carbonic Anhydrase I (rat) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL211
Anti-Carbonic Anhydrase II (mouse) Antibody, 100uL
100uL In stock
Regular Price:$321.00
ESL212
Anti-Carbonic Anhydrase I (mouse) Antibody, 100uL
100uL In stock
Regular Price:$321.00
Specifications

Product Type: Antibody
Comments: See "Comments" below for Specifications

Provider
From the laboratory of Abdul Waheed, PhD, St. Louis University.
Comments

Carbonic Anhydrase Antibodies

Antigen Name: CA-12
(human)
CA-4
(human)
CA-12
(mouse)
CA-14
(mouse)
CA-5A
(mouse)
CA-5B
(mouse)
CA-5A
(human)
CA-I
(rat)
CA-II
(mouse)
CA-I
(mouse)
Molecular Weight: 43-44 kDa 35 kDa 40-45 kDa 54 kDa 29 kDa 31 kDa 30-34 kDa 30 kDa 29-30 kDa 29-30 kDa
Immunoglobulin Isotype: IgG
Clonality: Polyclonal
Species Reactivity: Human Human Mouse Mouse, human Mouse Mouse Human Rat Mouse Mouse
Immunogen: Human recombinant CA XII Human recombinant CA IV (33-kD secretory form) Recombinant mouse CA XII Mouse recombinant CA XIV Mouse CA VA C-tail peptide Carboxyl terminal 17-aa CA VB peptide conjugated to thyroglobulin C-terminal 17 aa of CA V Rat CA I Protein purified from WT mouse RBC Protein purified from WT mouse RBC
Species Immunized: Rabbit
Antibody Purification Method: Total Serum
Antibody Concentration: Serum (~0.5mg-1.2mg/mL)
Method Used to Determine Concentration: Purified antibody (anti IgG purification of serum; protein A sepharose resin)
Final Buffer Concentration: Pure Serum
Storage Temperature: -80C
Shipped: Dry Ice
References
  1. Türeci Ö, Sahin U, Vollmar E, et al. Human carbonic anhydrase XII: cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(13):7608-7613.
  2. Parkkila S, Parkkila A-K, Rajaniemi H, et al. Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. Proceedings of the National Academy of Sciences of the United States of America. 2001;98(4):1918-1923.
  3. Parkkila, S., Parkkila, A., Juvonen, T., Waheed, A., Sly, W. S., Saarnio, J., Kaunisto, K., Kellokumpu, S. and Rajaniemi, H. (1996), Membrane-bound carbonic anhydrase IV is expressed in the luminal plasma membrane of the human gallbladder epithelium. Hepatology, 24: 1104–1108.
  4. Parkkila S, Rajaniemi H, Parkkila AK, Kivela J, Waheed A, Pastorekova S, Pastorek J, Sly WS. Carbonic anhydrase inhibitor suppresses invasion of renal cancer cells in vitro. Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2220-4.
  5. Halmi P, Lehtonen J, Waheed A, Sly WS, Parkkila S. Expression of hypoxia-inducible, membrane-bound carbonic anhydrase isozyme XII in mouse tissues. Anat Rec A Discov Mol Cell Evol Biol. 2004 Mar;277(1):171-7.
  6. Kyllönen MS, Parkkila S, Rajaniemi H, Waheed A, Grubb JH, Shah GN, Sly WS, Kaunisto K. Localization of carbonic anhydrase XII to the basolateral membrane of H+-secreting cells of mouse and rat kidney. J Histochem Cytochem. 2003 Sep;51(9):1217-24.
  7. Whittington DA, Grubb JH, Waheed A, Shah GN, Sly WS, Christianson DW. Expression, assay, and structure of the extracellular domain of murine carbonic anhydrase XIV: implications for selective inhibition of membrane-associated isozymes. J Biol Chem. 2004 Feb 20;279(8):7223-8.
  8. Shah GN, Ulmasov B, Waheed A, Becker T, Makani S, Svichar N, Chesler M, Sly WS. Carbonic anhydrase IV and XIV knockout mice: roles of the respective carbonic anhydrases in buffering the extracellular space in brain. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16771-6.
  9. Ochrietor JD, Clamp MF, Moroz TP, Grubb JH, Shah GN, Waheed A, Sly WS, Linser PJ. Carbonic anhydrase XIV identified as the membrane CA in mouse retina: strong expression in Müller cells and the RPE. Exp Eye Res. 2005 Oct;81(4):492-500.
  10. Shah GN, Rubbelke TS, Hendin J, Nguyen H, Waheed A, Shoemaker JD, Sly WS. Targeted mutagenesis of mitochondrial carbonic anhydrases VA and VB implicates both enzymes in ammonia detoxification and glucose metabolism. Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7423-8.
  11. Shah GN, Hewett-Emmett D, Grubb JH, Migas MC, Fleming RE, Waheed A, Sly WS. Mitochondrial carbonic anhydrase CA VB: differences in tissue distribution and pattern of evolution from those of CA VA suggest distinct physiological roles.
  12. Goldfarb DS, Sly WS, Waheed A, Charney AN. Acid-base effects on electrolytetransport in CA II-deficient mouse colon. Am J Physiol Gastrointest LiverPhysiol. 2000 Mar;278(3):G409-15.
  13. Fleming RE, Parkkila S, Parkkila AK, Rajaniemi H, Waheed A, Sly WS. Carbonicanhydrase IV expression in rat and human gastrointestinal tract regional,cellular, and subcellular localization. J Clin Invest. 1995 Dec;96(6):2907-13.
  14. Parkkila AK, Scarim AL, Parkkila S, Waheed A, Corbett JA, Sly WS. Expressionof carbonic anhydrase V in pancreatic beta cells suggests role for mitochondrial carbonic anhydrase in insulin secretion. J Biol Chem. 1998 Sep18;273(38):24620-3.

If you publish research with this product, please let us know so we can cite your paper.

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