Anti-Rabies Virus Nucleoprotein Antibodies
These mouse IgG monoclonal antibodies were generated against virus and are specific for the nucleoprotein of rabies virus.
Rabies is a viral disease that causes acute inflammation of the brain in humans and other warm-blooded animals. The rabies viral nucleoprotein encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, is termed the nucleocapsid (NC), and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases.
From the laboratory of Tadeusz J. Wiktor, DVM, The Wistar Institute.
These mouse IgG monoclonal antibodies were generated against virus and are specific for the nucleoprotein of rabies virus.
Rabies is a viral disease that causes acute inflammation of the brain in humans and other warm-blooded animals. The rabies viral nucleoprotein encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, is termed the nucleocapsid (NC), and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases.
From the laboratory of Tadeusz J. Wiktor, DVM, The Wistar Institute.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Antigen: | Rabies nucleocapsid protein |
| Accession ID: | Q8JXF6 |
| Isotype: | IgG |
| Clonality: | Monoclonal |
| Clone Name: | 806-1 |
| Reactivity: | Rabies virus |
| Immunogen: | Virus |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | IF, Virus neutralization and binding assays using ERA, Kelev, CVS-11 and HEP rabies viruses, all Nigerian strains (Ogunkoya, Beran and Wiktor, 2006 ) |
| Storage: | -20C |
| Shipped: | Cold packs |
Anti-Rabies Virus Nucleoprotein Antibodies
| Clone Name: | 806-1 | 808-2 | 804-9 | 807-5 |
| Antigen Name: | Rabies nucleocapsid protein | Rabies nucleocapsid protein | Rabies nucleocapsid protein | Rabies nucleocapsid protein |
| Immunogen Isotype: | IgG | IgG | IgG | IgG |
| Clonality: | Monoclonal | Monoclonal | Monoclonal | Monoclonal |
| Species Reactivity: | Rabies virus | Rabies virus | Rabies virus | Rabies virus |
| Recognized Strains: | ERA, Kelev, CVS-11 and HEP rabies viruses, all Nigerian strains (Ogunkoya, Beran and Wiktor, 2006 ) | ERA, Kelev, CVS-11 and HEP and several street rabies strains (Lafon and Wiktor, 1985), and some Nigerian Rabies strains (Ogunkoya, Beran and Wiktor, 2006 ) | ERA, Kelev, CVS-11 and HEP rabies viruses, all Nigerian strains (Ogunkoya, Beran and Wiktor, 2006 ) | ERA, Kelev, CVS-11 and HEP rabies viruses, all Nigerian strains (Ogunkoya, Beran and Wiktor, 2006 ) |
| Species Immunized: | Mouse | Mouse | Mouse | Mouse |
| Immunogen: | Virus | Virus | Virus | Virus |
| Purification Method: | Protein G | Protein G | Protein G | Protein G |
| Storage Buffer: | 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide | |||
| Tested Applications: | IF, Virus neutralization and binding assays | IF, Virus neutralization and binding assays | IF, Virus neutralization and binding assays | IF, Virus neutralization and binding assays |
| Storage Temperature: | -20C | -20C | -20C | -20C |
| Shipped: | Cold packs | Cold packs | Cold packs | Cold packs |
- Ogunkoya AB, Beran GW, Wiktor TJ. Antigenic analysis of some Nigerian street rabies virus using monoclonal antibodies. Nigerian Veterinary Journal Vol. 27 (2) 2006: pp. 41-53.
- Lafon M, Wiktor TJ. Antigenic sites on the ERA rabies virus nucleoprotein and non-structural protein. J Gen Virol. 1985 Oct;66 ( Pt 10):2125-33.
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