Miami Orange is a DNA-binding fluorescent probe that allows for two-channel imaging of nuclear and cytosolic compartments.
The Miami Orange and Miami Red dyes are DNA-targeting probes that exhibit binding-induced ‘turn on’ fluorescence. While a ‘turn-on’ response can result through interaction with RNA or any number of protein binding folds present in the cytosolic milieu, these probes bound to DNA exhibit redder excitation and emission spectra, enabling clear resolution of the nucleus. These dyes effectively combine the function of two separate dyes (i.e., a nuclear stain and a cytosolic stain) in a single probe.
From the laboratory of James N. Wilson, PhD, University of Miami.
Part of The Investigator's Annexe program.
|Product Type:||Small Molecule|
|Name:||Miami Orange ("1" in reference)|
|Format:||orange powder (neat), orange liquid (as shipped)|
|Purity:||> 95% by 1HNMR|
|Solubility:||Soluble in DMSO up to 1.5mM, soluble in aqueous solutions up to 5uM|
|Spectral Information:||λ max, ex (DNA bound) = 454nm, λ max, em = 582nm|
|Platform:||Confocal microscope, Fluorometer|
|Compatible Cells:||MCF7, BT474 (others not tested)|
|Comments:||Suggested working concentration 2-5uM|
|Storage:||4C (protect from light)|
(A) Excitation and emission spectra of Miami Orange. (B) Representative image of cells (HEK293) treated with Miami Orange.
If you publish research with this product, please let us know so we can cite your paper.