3T3 L1 and 3T3 F442A are clonal sublines isolated from 3T3 mouse embryonic fibroblasts, and can be differentiated to adipocytes. These cell lines are commonly used a model to study fat metabolism.
Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes. Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.
From the laboratory of Howard Green, MD, Harvard University.
Part of The Investigator's Annexe program.
|Product Type:||Cell Line|
|Name:||3T3 L1 & 3T3 F442A|
|Cell Type:||Embryonic mouse fibroblast preadipose cell line|
|Accession ID:||L1, CVCL_0123; F442A, CVCL_0122|
|Morphology:||Fibroblastic, can be differentiated to adipocytes|
|Growth Conditions:||Cells grow in 10% CO2 at 37C in a humidified incubator. Media: DMEM (high glucose, no sodium pyruvate and no HEPES) supplemented with 10% bovine calf serum; penn/strep and 2mM L-Glutamine may be added. DO NOT use fetal bovine serum as this will cause increased background differentiation. DO NOT use fetal bovine serum. Growth in fetal bovine serum affects the growth potential of these cells. Bovine calf serum should be iron supplemented and NOT gamma irradiated or heat inactivated (Recommended serum: GE Healthcare Iron-Supplemented BVN CLF 500ml; Cat.SH30072.03).|
|Subculturing:||Subculture the cells once they reach 60-80% confluency It is very important not to let the cells get confluent or they will begin to spontaneously differentiate. Split the cells at a density of ~ 3.3 x 103cells/cm2. Feed every 2 to 3 days with complete medium.|
|Cryopreservation:||Complete media with 10% sterile DMSO|
When thawing these cells, do not centrifuge, as this can lead to a poor recovery rate. The providing laboratory recommends:
To differentiate 3T3-F442A cells to adipocytes see: Djian, P., Phillips, M., and Green, H. (1985). The activation of specific gene transcription in the adipose conversion of 3T3 cells. J Cell Physiol 124, 554-6. The 3T3-F442A line should be 80-90% differentiated after 2 weeks in FBS and insulin. 3T3-L1 differentiation is less extensive than that of 3T3-F442A under the same conditions.
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