3T3 L1 and 3T3F442A are clonal sublines isolated from 3T3 mouse embryonic fibroblasts, and can be differentiated to adipocytes. These cell lines are commonly used a model to study fat metabolism.
Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes. Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.
From the laboratory of Howard Green, MD, Harvard University.
Part of The Investigator's Annexe program.
|Product Type:||Cell Line|
|Name:||3T3 L1 & 3T3 F442A|
|Cell Type:||Embryonic mouse fibroblast preadipose cell line|
|Accession ID:||L1, CVCL_0123; F442A, CVCL_0122|
|Morphology:||Fibroblastic, can be differentiated to adipocytes|
|Subculturing:||Subculture the cells once they reach 60-80% confluency It is very important not to let the cells get confluent or they will begin to spontaneously differentiate. Split the cells at a density of ~ 3.3 x 103cells/cm2. Feed every 2 to 3 days with DMEM supplemented with 10% bovine calf serum. DO NOT use fetal bovine serum as this will cause increased background differentiation.|
|Growth Conditions:||Cells grow in 10% CO2 at 37C in a humidified incubator. Media: DMEM supplemented with 10% bovine calf serum. DO NOT use fetal bovine serum as this will cause increased background differentiation.|
|Cryopreservation:||DMEM with 10% bovine calf serum containing 10% sterile DMSO|
To differentiate 3T3-F442A cells to adipocytes see: Djian, P., Phillips, M., and Green, H. (1985). The activation of specific gene transcription in the adipose conversion of 3T3 cells. J Cell Physiol 124, 554-6. The 3T3-F442A line should be 80-90% differentiated after 2 weeks in FBS and insulin. 3T3-L1 differentiation is less extensive than that of 3T3-F442A under the same conditions.
If you publish research with this product, please let us know so we can cite your paper.