Recombinant full length (aa 1-260) Human Deoxycytidine kinase (dCK) variant (R104M, D133A) purified by nickel-sepharose chromatography.
Deoxycytidine kinase (dCK, EC:188.8.131.52) is required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG), and deoxyadenosine (dA). dCK has a broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
Also available: Wild-type Human Deoxycytidine Kinase (dCK)
From the laboratory of Arnon Lavie, PhD, University of Illinois at Chicago.
Part of The Investigator's Annexe program.
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|Name:||Human dCK (R104M, D133A) (dCK-DM)|
|Accession ID:||P27707 mutated at positions 104 and 133|
|Source:||Recombinant expression in E. coli|
|Molecular Weight:||~31 kDa|
|Amino Acid Sequence:||MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLS (R>M)IRAQLASLNGKLKDAEKPVLFFERSVYS(D>A)RYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL|
|Fusion Tag(s):||N-terminal His tag|
|Buffer:||25 mM Tris pH7.5, 500 mM NaCl, 20 % glycerol, 10 mM DTT, 1 mM EDTA|
|Activity:||? 145 IU/mg protein; kcat 1.22/sec with dC as substrate; One unit of WT human dCK converts 1.0 µmole of dC and ATP to dCMP and ADP per minute at pH 7.5 at 37°C, as measured by a coupled enzyme system with 200 µM dC and 1 mM ATP.|
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