KAZU DNA Extraction Buffer

The KAZU DNA Extraction Buffer allows for the rapid extraction of genomic DNA from plant leaves for the downstream amplification of targets of interest by PCR.

Highlights:

  • Quick - 10 minutes per prep
  • Great for many applications: PCR, qPCR, inverse PCR
  • Ideal for high throughput (plate-based) applications
  • Nontoxic and stable - Does not contain phenol or chloroform, and can be used to store samples long term

DNA extraction is typically performed using buffers such as a CTAB or PVP, or kits that often contain toxic ingredients such as phenol and chloroform, require a long time commitment (over 3 hours), and require the use of many tubes and kits. Non-phenol and non-chloroform Column-based DNA extraction kits are available for rapid extraction (less than 1 hour) but these kits are expensive. The KAZU DNA Extraction Buffer offers a non toxic solution that takes only 10 minutes per reaction, and is compatible with high throughput methods.

From the laboratory of Thomas P. Brutnell, PhD, Donald Danforth Plant Science Center.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EDD001
KAZU DNA Extraction Buffer, 1mL (Trial Size)
1mL (Trial Size) In stock
Regular Price:$26.00
EDD002
KAZU DNA Extraction Buffer, 10 mL (100 reactions)
10mL (100 rxns) In stock
Regular Price:$51.00
EDD003
KAZU DNA Extraction Buffer, 50mL (500 reactions)
50mL (500 rxns) In stock
Regular Price:$205.00
Specifications

Product Type: Buffer or Chemical
Format: Non-column based
Components: KAZU DNA Extraction Buffer
Elution Volume: 100 uL per sample
Sample Type: Plant leaf and seed (animal tissue likely, but not tested)
Technology: High efficiency and quick ethanol precipitation
Time per prep: 10 min
Yield: 500 ng
Tested Applications: DNA extraction from tissues for downstream PCR, qPCR, inverse PCR
Amount: 1/4" hole punch
Storage: Room temperature
Shipped: Ambient temperature

Documentation

PDF Suggested Protocol

Comparative Data

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Provider
From the laboratory of Thomas P. Brutnell, PhD, Donald Danforth Plant Science Center.
References
  1. Zhou S, Kremling KA, Bandillo N, Richter A, Zhang YK, Ahern KR, Artyukhin AB, Hui JX, Younkin GC, Schroeder FC, Buckler ES, Jander G. Metabolome-Scale Genome-Wide Association Studies Reveal Chemical Diversity and Genetic Control of Maize Specialized Metabolites. Plant Cell. 2019 May;31(5):937-955. View Article

If you publish research with this product, please let us know so we can cite your paper.

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