The KAZU DNA Extraction Buffer allows for the rapid extraction of genomic DNA from plant leaves for the downstream amplification of targets of interest by PCR.
DNA extraction is typically performed using buffers such as a CTAB or PVP, or kits that often contain toxic ingredients such as phenol and chloroform, require a long time commitment (over 3 hours), and require the use of many tubes and kits. Non-phenol and non-chloroform Column-based DNA extraction kits are available for rapid extraction (less than 1 hour) but these kits are expensive. The KAZU DNA Extraction Buffer offers a non toxic solution that takes only 10 minutes per reaction, and is compatible with high throughput methods.
From the laboratory of Thomas P. Brutnell, PhD, Donald Danforth Plant Science Center.
Part of The Investigator's Annexe program.
|Product Type:||Buffer or Chemical|
|Components:||KAZU DNA Extraction Buffer|
|Elution Volume:||100 uL per sample|
|Sample Type:||Plant leaf and seed (animal tissue likely, but not tested)|
|Technology:||High efficiency and quick ethanol precipitation|
|Time per prep:||10 min|
|Tested Applications:||DNA extraction from tissues for downstream PCR, qPCR, inverse PCR|
|Amount:||1/4" hole punch|
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