This fluorescent nuclear dye has been shown to be brighter than traditional nuclear stains (e.g., DAPI, Hoechst) in Human Embryonic Kidney (HEK) and breast cancer cells (MCF7). It is ideal for in vivo cell or tissue studies where prolonged imaging experiments are necessary.
From the laboratory of James N. Wilson, PhD, University of Miami.
Part of The Investigator's Annexe program.
|Product Type:||Small Molecule|
|Name:||Miami Yellow ("2" in reference)|
|Purity:||> 95% by 1HNMR|
|Solubility:||Soluble in DMSO up to at least 1.5mM, soluble in aqueous solutions up to 5uM|
|Spectral Information:||λ max, ex (DNA bound) = 502nm, λ max, em = 558nm|
|Platform:||Confocal microscope, Fluorometer|
|Compatible Cells:||HEK293, MCF7, BT474 (others not tested)|
|Comments:||Suggested working concentration 1-2uM|
|Storage:||4C (protect from light)|
(A) Excitation and emission spectra of Miami Yellow. (B) Representative image of cells (MCF7) treated with Miami Yellow.