This polyphosphate (Medium Chain, p100) is heterogeneous in size, with approximate polymer lengths ranging from ~45-160 phosphate units; modal size is about 75 phosphate units.
Polyphosphate, a linear polymer of inorganic orthophosphate, which is widespread in living organisms and which plays diverse roles in biology, has recently been shown to be a potent modulator of the human blood clotting system. Its procoagulant activity is dependent on polymer length. This medium chain polyphosphate (p100) is very similar size distribution to natural polyphosphates secreted by platelets.
In addition to being a useful tool for plasma clotting assays, polyphosphate is can be used to study bacterial growth, virulence, and bacterial membrane function.
From the laboratory of James H. Morrissey, PhD, University of Michigan.
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|Product Type:||Small Molecule|
|Name:||Polyphosphate, Medium Chain (p100; biotinylated)|
|Chemical Formula:||Nan+2PnO3n +1 n = the average number of phosphorus (P) atoms in the chain|
|Molecular Weight:||Heterogeneous; Number average ~11.5 kDa; Range ~2 kDa to 28.5 kDa|
|Buffer:||20 mM Hepes-NaOH pH 7.4, 5 mM EDTA|
|Solubility:||At least 100 mM in water; <1 mM in buffers containing divalent metals (make intermediate dilutions in divalent metal ion free buffers)|
|Concentration:||When reconstituted: 1 M (phosphate monomer) NOTE: To estimate the polymer concentration, divide the phosphate monomer concentration by the modal polymer length|
|Size Distribution:||Mode of n: 75 (as measured by migration on PAGE)|
|Comments:||It is recommended to use ~10.2 ug of the biotin-polyphosphate to coat all wells of a streptavidin-coated 96-well plate.|
Dry material should be stored at room temperature (or lower) with dessication.
- Stable at least 12 hours at room temperature
- Stable at least 72 hours at 4C
- Store long-term at -80C
- Aliquot for repeated use, avoiding repeated freeze-thaw cycles
- Do not store in the presence of divalent metal ions
Polyphosphate has limited solubility in the presence of divalent metal ions, so make intermediate dilutions in water, or in buffers without divalent metals or polyphosphate-binding proteins.
1 - Centrifuge 100mg tube 1 min at 1000 x g to pellet material in bottom of tube.
2 - Reconstitute in 1.0 mL purified water to make a solution of 1 M phosphate (= 102 mg/mL polyphosphate).
NOTE: May be reconstituted in larger volumes to make a more dilute solution as needed.
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