An alkyne-activated desthiobiotin labeling reagent with a hydrophilic spacer arm.


  • Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
  • Desthiobiotin – allows easy elution from streptavidin, an ideal feature for affinity purification applications
  • Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
  • Extended PEG3 spacer – the PEG spacer arm is hydrophilic and provides labeled molecules with greater solubility to compared to reagents having only hydrocarbon spacers

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Desthiobiotin-PEG4-Alkyne, 25 mg
25mg In stock
Regular Price:$480.00
On Sale:
Desthiobiotin-PEG4-Alkyne, 100 mg
100mg In stock
Regular Price:$1,412.00
On Sale:

Product Type: Small Molecule
Name: Desthiobiotin-PEG4-Alkyne
Chemical Formula: C21H37N3O6
Molecular Weight: 427.23
Variant MPN: 1109
Format: Colorless to slightly yellow oil
Solubility: DMSO, DMF, MeOH
Storage: -20C
Shipped: Ambient


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