TAMRA-Azide-Biotin

A trifunctional biotinylation reagent that can be used for both visualization and affinity purification of alkyne-tagged molecules.

Highlights:

  • Trifunctional – allows for both visualization and affinity purification of alkyne-tagged proteins
  • Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
  • Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures

TAMRA-Azide-Biotin incorporates an azido group to enable click chemistry-mediated labeling of alkyne-tagged proteins. A hydophilic PEG spacer increases solubility in aqueous media and reduces steric hindrance when binding to streptavidin molecules. The biotin enables efficient protein enrichment. Finally, a carboxytetramethylrhodamine (TAMRA) fluorophore, which remains attached to the protein after elution from streptavidin resin, serves the purpose of sensitive detection of tagged proteins.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
FCC332
TAMRA-Azide-Biotin, 5 mg
5mg In stock
Regular Price:$435.00
On Sale:
FCC333
TAMRA-Azide-Biotin, 25 mg
25mg In stock
Regular Price:$1,450.00
On Sale:
Specifications

Product Type: Small Molecule
Name: TAMRA-Azide-Biotin
Chemical Formula: C57H79N11O14S
Molecular Weight: 1174.37
Extinction Coefficient: 92000 M-1cm-1
Variant MPN: 1048
Format: Red amorphous solid
Solubility: DMSO, DMF, water, MeOH
Spectral Information: Abs/Em = 546/565 nm
Storage: Upon receipt store at -20C
Shipped: Ambient temperature

Documentation
References

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