Diazo Biotin-Azide

An alkyne-reactive, sodium dithionite (Na2S2O4) cleavable biotinylation reagent.


  • Cleavable – a diazo spacer arm allows protein targets to be released under mild conditions
  • Chemoselective – azide and alkyne groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
  • Solubility – reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
  • Extended PEG3 spacer – reduces aggregation, minimizes steric hindrance for affinity-binding to avidin or streptavidin

Diazo Biotin-Azide allows the capture of alkyne-tagged biomolecules via a copper-catalyzed click reaction. This reaction possesses extreme selectivity, such that the complementary reagents can form covalent bonds within richly functionalized biological systems, and, in some cases, living organisms. Biotinylated protein targets are selectively captured through conventional affinity purification protocols that exploit the biotin - streptavidin interaction. Captured protein targets can be release of under mild conditions with 50 mM sodium dithionite solution, and identified by mass spectrometry, ELISA, dot blot, or Western blot applications.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Diazo Biotin-Azide, 25 mg
25mg In stock
Regular Price:$779.00
On Sale:

Product Type: Small Molecule
Name: Diazo Biotin-Azide
Chemical Formula: C33H45N9O7S
Molecular Weight: 711.83
Variant MPN: 1041
Format: Orange solid to grey solid
Solubility: DMSO, DMF
Storage: Upon receipt store at -20C
Shipped: Ambient temperature


If you publish research with this product, please let us know so we can cite your paper.