DBCO-Diazo Biotin
A sodium dithionite (Na2S2O4) cleavable biotinylation reagent for selective enrichment of azide-tagged biopolymers via SPAAC reaction.
Highlights:
- Cleavable – a diazo spacer arm allows biotinylated protein targets to be released under mild conditions
- Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
- Soluble – the reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
- Extended PEG3 spacer – reduces aggregation, minimizes steric hindrance for affinity-binding to avidin or streptavidin
DBCO-Diazo Biotin allows capture of azide-tagged biomolecules via the Cu-free click reaction under mild aqueous buffer conditions. This reaction possesses extreme biocompatibility, such that the complementary reagents can form covalent bonds within living organisms. Biotinylated protein targets can be selectively captured through conventional affinity purification protocols that exploit the biotin - streptavidin interaction. Captured protein targets can be released under mild conditions with 50 mM sodium dithionite solution, and identified by mass spectrometry, ELISA, dot blot, or Western blot applications.
A sodium dithionite (Na2S2O4) cleavable biotinylation reagent for selective enrichment of azide-tagged biopolymers via SPAAC reaction.
Highlights:
- Cleavable – a diazo spacer arm allows biotinylated protein targets to be released under mild conditions
- Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
- Soluble – the reagent easily dissolves in water-miscible solvents for subsequent dilution into aqueous reaction mixtures
- Extended PEG3 spacer – reduces aggregation, minimizes steric hindrance for affinity-binding to avidin or streptavidin
DBCO-Diazo Biotin allows capture of azide-tagged biomolecules via the Cu-free click reaction under mild aqueous buffer conditions. This reaction possesses extreme biocompatibility, such that the complementary reagents can form covalent bonds within living organisms. Biotinylated protein targets can be selectively captured through conventional affinity purification protocols that exploit the biotin - streptavidin interaction. Captured protein targets can be released under mild conditions with 50 mM sodium dithionite solution, and identified by mass spectrometry, ELISA, dot blot, or Western blot applications.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Small Molecule |
| Name: | DBCO-Diazo Biotin |
| Chemical Formula: | C52H60N8O9S |
| Molecular Weight: | 973.15 |
| Variant MPN: | 1043 |
| Format: | Orange solid |
| Solubility: | DMSO, DMF |
| Storage: | -20C. Desiccate |
| Shipped: | Ambient |
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