A sodium dithionite (Na2S2O4) cleavable biotinylation reagent for selective enrichment of azide-tagged biopolymers via SPAAC reaction.
DBCO-Diazo Biotin allows capture of azide-tagged biomolecules via the Cu-free click reaction under mild aqueous buffer conditions. This reaction possesses extreme biocompatibility, such that the complementary reagents can form covalent bonds within living organisms. Biotinylated protein targets can be selectively captured through conventional affinity purification protocols that exploit the biotin - streptavidin interaction. Captured protein targets can be released under mild conditions with 50 mM sodium dithionite solution, and identified by mass spectrometry, ELISA, dot blot, or Western blot applications.
|Product Type:||Small Molecule|
|Storage:||Upon receipt store at -20C|
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