DBCO-PEG5-NHS Ester
An amine-reactive labeling reagent with enhanced water stability and solubility.
Highlights:
- Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
- Chemoselective – azides and DBCO groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Primary amine reactivity – modifies amine-containing molecules with DBCO for use in advanced crosslinking experiments
- Extended PEG5 spacer – reduces aggregation, minimizes steric hindrance, and enhances solubility
DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.
An amine-reactive labeling reagent with enhanced water stability and solubility.
Highlights:
- Biocompatible – click reaction occurs efficiently under mild buffer conditions; requires no accessory reagents such as a copper catalyst or reducing agents (e.g. DTT)
- Chemoselective – azides and DBCO groups do not react or interfere with other functional groups found in biological samples, but conjugate to one another with high efficiency
- Primary amine reactivity – modifies amine-containing molecules with DBCO for use in advanced crosslinking experiments
- Extended PEG5 spacer – reduces aggregation, minimizes steric hindrance, and enhances solubility
DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
Specifications
| Product Type: | Small Molecule |
| Name: | DBCO-PEG5-NHS Ester |
| Chemical Formula: | C36H43N3O11 |
| Molecular Weight: | 693.74 |
| Variant MPN: | A102P |
| Format: | Yellow to slightly orange oil |
| Solubility: | DMSO, DMF, DCM, THF, CHCl3 |
| Storage: | -20C. Desiccate |
| Shipped: | Frozen overnight - Ambient |
Documentation
References
- Wang G, Moffitt JR, Zhuang X. Multiplexed imaging of high-density libraries of RNAs with MERFISH and expansion microscopy. Sci Rep. 2018 Mar 19;8(1):4847. View Article
- If you publish research with this product, please let us know so we can cite your paper.
