This three-dimensional (3-D) semi-solid media contains the necessary components for the differentiation of dissociated single pancreatic cells into insulin-producing cells from murine adult and postnatal samples.
From the laboratory of H. Teresa Ku, PhD, City of Hope.
Part of The Investigator's Annexe program.
|Buffer or Chemical
|Pancreatic Cell Differentiation Medium
|Phenol Red Indicator:
|Soluble and conditioned media with growth factors and viscous materials
|4C, do not freeze. Use within 2 weeks of receiving
Representative photomicrograph of insulin-expressing "ring" colonies in semi solid culture
Colonies appear as circles when viewed under a phase-contrast microscope, but are in fact cystic in 3D space. Individual colonies can later be assayed for gene and protein expression by RT-PCR and immunohistochemistry analyses.
IMPORTANT: Be sure to use within 2 weeks of receiving material
For detailed information on plating of cells and counting of the resulting colonies, please refer to Winkler M., et al. J Vis Exp. 2011 Nov 28;(57):e3148.
For information on dissociation of adult murine pancreas, please see Jin et al. Proc Natl Acad Sci U S A 110:3907-3912 (2013).
NOTE: Up to 25,000 cells can be plated per well in a 24-well dish. However, optimal plating density of cells will vary based of cell populations and should be tested by individual laboratories.
If you publish research with this product, please let us know so we can cite your paper.