This biotinylated bacterial protein can be used as a procedural control for affinity capture experiments using avidin or streptavidin beads and biotinylated proteins.
Avidin and streptavidin are widely used in biotechnology and molecular research for detection, purification, crosslinking, and labeling of biotinylated targets. This protein can be used in a manner similar to the way prebiotinylated AhpC was used in experiments with DCP-Bio1 (discussed in Klomsiri et al., Methods Enzymol, 2010).
From the laboratory of Leslie B. Poole, PhD, Wake Forest School of Medicine.
Part of The Investigator's Annexe program.
|Name:||Biotinylated-Trx Loading Control Protein|
|Accession ID:||P0AA25 (THIO_ECOLI)|
|Source:||Recombinantly expressed in E.Coli and modified with biotin-maleimide|
|Molecular Weight:||11,675 + 903 Da (2 biotin molecules)|
|Amino Acid Sequence:||SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA|
|Purity:||95% as determined by SDS-PAGE|
|Buffer:||25mM potassium phosphate pH 7, 1mM EDTA|
|Suggested Amount per Experiment:||1 microgram per 1000 micrograms of sample|
|Comments:||Has 2 biotin-maleimide moieties attached at Cys residues|
|Storage:||-20C (avoid repeated freeze-thaw cycles)|
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