DCP-Alkyne

DCP-Alkyne is dimedone based and contains an alkyne group allowing for selective conjugation via “click” chemistry or Staudinger ligation to azide linked detectable reagents such as biotin or common fluorophores.

Highlights:

  • Stable, reproducible binding to cysteine sulfenic acid (-SOH) at pH 6.0-8.0
  • Highly customizable - Alkyne group allows for use in a variety of analytical techniques
  • Great for in vitro and in vivo applications
  • Compatible with WB, ELISA, and Affinity Isolation

Redox-sensitive cysteine residues in proteins may serve as important components of oxidative signaling or sensors of oxidative stress. Cysteine sulfenic acid modification is an emerging area of interest for those studying biological signal transduction within the cell.

Cysteine sulfenic acid formation in proteins results from the oxidative modification of susceptible cysteine residues by mild oxidizing agents such as hydrogen peroxide, alkyl hydroperoxides, and peroxynitrite. These sulfenic acid modified proteins can be identified by their ability to form adducts with dimedone, but this reagent provides no spectral or affinity tag to such adduct to allow for later analysis. DCP-Alkyne can be used to effectively detect the formation of cysteine sulfenic acid in the redox regulation of proteins. The alkyne group can be used for selective conjugation to phosphine- or alkynyl- containing reagents such as biotin or common fluorophores for desired analytical techniques, and can be used to label protein sulfenic acids in cellular proteins, either by in situ labeling of intact cells or by labeling at the time of lysis.

From the laboratories of S. Bruce King, PhD and Leslie B. Poole, PhD, Wake Forest School of Medicine.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EE0032
DCP-Alkyne, 4mg
4mg (2x2mg) In stock
Regular Price:$123.00
On Sale:
EE0033
DCP-Alkyne, 10mg
10mg In stock
Regular Price:$267.00
On Sale:
Specifications

Product Type: Small Molecule
Name: DCP-Alkyne (aka DYn-2)
Chemical Formula: C11H14O2
Source: Synthetic
Molecular Weight: 178.2 g/mol
Format: off-white solid
Purity: >95% by NMR
Solubility: DMSO, ethanol, 50% ethanol (up to 100 mg/ml)
Storage: -20C for long term storage
Shipped: Ambient temperature

Provider
From the laboratories of S. Bruce King, PhD and Leslie B. Poole, PhD, Wake Forest School of Medicine.
References
  1. Paulsen, C.E., Truong, T.H., Garcia, F.J., Homann, A., Gupta, V., Leonard, S.E. & Carroll, K.S. Peroxide-dependent sulfenylation of the EGFR catalytic site enhances kinase activity. Nat Chem Biol 8, 57-64 (2011).
  2. Wages PA. Detecting Protein Sulfenylation in Cells Exposed to a Toxicant. Curr Protoc Toxicol. 2017 Feb 1;71:17.18.1-17.18.12. View Article

If you publish research with this product, please let us know so we can cite your paper.

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