This premade lentivirus includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction and expression of the target genes as well as antibiotics resistant genes or reporters. A woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) is believed to promote RNA processing events and nuclear export, boosting expression of your gene of interest in target cells by facilitating the production of mature mRNA from transcripts initiated by the virus's internal promoter (e.g., pCMV).
|Vector Information:||Vector includes both 5? and 3? lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)|
|Target Gene / Reporter(s):||GFP|
|Virus:||GFP (Inducible Tet-On) Lentivirus|
|Titer:||1 x 10^8TU/mL|
|Storage:||Store at -80C. Multiple freeze/thaw cycles not recommended.|
NOTE: This Inducible Tet-On lentivirus does not contain the reverse tetracycline-controlled transactivator (rtTA) protein, which is required for the Tet-On system. Unless the cells used stably produce rtTA, it is necessary to cotransduce this lentivirus with a rtTA lentivirus (rtTA (CMV, neomycin) Lentivirus or rtTA (CMV, puromycin) Lentivirus.
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