This mouse monoclonal antibody was generated against a ΦX174 bacteriophage-derived synthetic DNA–RNA antigen and recognizes RNA-DNA hybrids of various lengths.
Recombinant Versions Available:
DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. Because RNA-DNA hybrids influence genomic instability, the S9.6 antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, the S9.6 antibody is effective in recognizing RNA-DNA hybridization for microarray studies.
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
|Antigen:||S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen|
|Isotype:||Mouse IgG2a, rabbit IgG, mouse Fab, goat IgG|
|Fusion Tag(s):||Mouse Fab version contains His-tag|
|Reactivity:||High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids|
|Immunogen:||ΦX174 bacteriophage-derived synthetic DNA/RNA|
|Species Immunized:||BALB/c mouse|
|Purification Method:||Protein A/G|
ENHOO1: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Ab01137- : PBS with 0.02% Proclin 300
Dot Blot Analysis: 0.2 µg/mL.
|Storage:||-20C (avoid repeated freeze-thaw cycles)|
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