This hybridoma line constitutively produces a mouse anti-Aspergillus fumigatus monoclonal antibody (clone 16C4) specific to the apical region.
Aspergillus fumigatus is a widespread saprobe that breaks down organic material in soil. The conidia of this fungus are ubiquitous in the atmosphere and commonly inhaled. In healthy individuals, A. fumigatus can cause allergic reactions or localized infections. In the immunocompromised, however, it can cause invasive aspergillosis, an increasingly frequent and often fatal diease.
From the laboratory of Michelle Momany, PhD, University of Georgia
Part of The Investigator's Annexe program.
|Cell Type:||Hybridoma cell line|
|Antigen:||A. fumigatus cell wall|
|Immunogen:||A. fumigatus237 isolated cell walls|
|Growth Conditions:||IMDM + 20% FBS + 1% Pen/Strep|
|Cryopreservation:||Resuspend cells in 90% MDM, 10% DMSO. Store vials at -80C for 24-48 hrs at -70 before transfer to liquid nitrogen.|
|Epitope:||Apical protein ~70kDa, no septa|
|Tested Applications:||ELISA (1:10), IF (1:10), IEM (1:10), WB (neat)|
mAb16C4 labels areas of polar growth.
A. fumigatus 237 was incubated for (a) 5, (b) 6, (c) 7, (d) 8 or (e) 9 h, fixed and labeled with mAb16C4 followed by FITC-conjugated anti-mouse secondary antibody. Left column, DIC image; right column, FITC image. Bars: 5 mm (ac); 15 mm (d, e).
mAb16C4 recognizes a protein of ~70 kDa.
Total protein (lanes 2, 4) and the positive fraction from the Q column (lanes 1, 3) were analysed by PAGE, silver-stained (lanes 1, 2), Western blotted and hybridized with mAb16C4 (lanes 3, 4).
Adapted from: Momany, M., et al. (2004) Microbiology 150, 3261-8.
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