Anti-Aspergillus fumigatus [8A1] Antibody

This hybridoma line constitutively produces a mouse anti-Aspergillus fumigatus monoclonal antibody (clone 8A1) specific to the conidium and basal region.

Highlights:

  • Reacts with Aspergillus fumigatus
  • Suitable for ELISA and Immunofluorescence applications

Recombinant versions available at our sister company, Absolute Antibody:

Aspergillus fumigatus is a widespread saprobe that breaks down organic material in soil. The conidia of this fungus are ubiquitous in the atmosphere and commonly inhaled. In healthy individuals, A. fumigatus can cause allergic reactions or localized infections. In the immunocompromised, however, it can cause invasive aspergillosis, an increasingly frequent and often fatal diease.

From the laboratory of Michelle Momany, PhD, University of Georgia

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA107
Anti-Aspergillus fumigatus [8A1] Hybridoma, 1 vial
1 vial In stock
Regular Price:$913.00
On Sale:
EGA122
Anti-Aspergillus fumigatus [8A1] Antibody (supernatant), 5mL
5mL (supernatant) In stock
Regular Price:$269.00
On Sale:
Specifications

Product Type: Antibody
Cell Type: Hybridoma cell line
Antigen: A. fumigatus cell wall
Isotype: IgM
Clonality: Monoclonal
Clone Name: 8A1
Reactivity: Aspergillus fumigatus
Immunogen: A. fumigatus237 isolated cell walls
Growth Conditions: IMDM + 20% FBS + 1% Pen/Strep
Cryopreservation: Resuspend cells in 90% MDM, 10% DMSO. Store vials at -80C for 24-48 hrs at -70 before transfer to liquid nitrogen.
Species Immunized: Mouse
Epitope: Conidium and basal, no septa
Tested Applications: ELISA , IF 1:10
Storage: Liquid nitrogen
Shipped: Dry ice

Data

A. fumigatus mAbs show distinct localization patterns

A. fumigatus 237 was grown for 12–14 h, fixed, incubated with primary mAb, followed by FITC-conjugated anti-mouse secondary antibody, Calcofluor White to label chitin and Hoechst 33258 to label nuclei. Primary antibodies: mAb1E2 (a), mAb8A1 (b) and mAb19H1 (c). Left column, FITC; right column, chitinous cell wall and nuclei. Arrows mark conidia; arrowheads mark septa; the double arrowhead marks a newly formed branch from main hypha.

Adapted from: Momany, M., et al. (2004) Microbiology 150, 3261-8.

Provider
From the laboratory of Michelle Momany, PhD, University of Georgia
References
  1. Ste-Marie L, Sénéchal S, Boushira M, Garzon S, Strykowski H, Pedneault L, de Repentigny L. Production and characterization of monoclonal antibodies to cell wall antigens of Aspergillus fumigatus. Infect Immun. 1990 Jul;58(7):2105-14.
  2. Momany M, Lindsey R, Hill TW, Richardson EA, Momany C, Pedreira M, Guest GM, Fisher JF, Hessler RB, Roberts KA. The Aspergillus fumigatus cell wall is organized in domains that are remodelled during polarity establishment. Microbiology. 2004 Oct;150(Pt 10):3261-8.

If you publish research with this product, please let us know so we can cite your paper.

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