This hybridoma line constitutively produces a mouse anti-Aspergillus fumigatus monoclonal antibody (clone 1E2) specific to the apical region of hyphae.
Aspergillus fumigatus is a widespread saprobe that breaks down organic material in soil. The conidia of this fungus are ubiquitous in the atmosphere and commonly inhaled. In healthy individuals, A. fumigatus can cause allergic reactions or localized infections. In the immunocompromised, however, it can cause invasive aspergillosis, an increasingly frequent and often fatal diease.
From the laboratory of Michelle Momany, PhD, University of Georgia
Part of The Investigator's Annexe program.
|Cell Type:||Hybridoma cell line|
|Antigen:||A. fumigatus cell wall|
|Clone Name:||1 E2|
|Immunogen:||A. fumigatus237 isolated cell walls|
|Growth Conditions:||IMDM + 20% FBS + 1% Pen/Strep|
|Cryopreservation:||Resuspend cells in 90% MDM, 10% DMSO. Store vials at -80C for 24-48 hrs at -70 before transfer to liquid nitrogen.|
|Epitope:||Apical, no septa|
|Tested Applications:||ELISA , IF 1:10|
A. fumigatus mAbs show distinct localization patterns
A. fumigatus 237 was grown for 1214 h, fixed, incubated with primary mAb, followed by FITC-conjugated anti-mouse secondary antibody, Calcofluor White to label chitin and Hoechst 33258 to label nuclei. Primary antibodies: mAb1E2 (a), mAb8A1 (b) and mAb19H1 (c). Left column, FITC; right column, chitinous cell wall and nuclei. Arrows mark conidia; arrowheads mark septa; the double arrowhead marks a newly formed branch from main hypha.
Adapted from: Momany, M., et al. (2004) Microbiology 150, 3261-8.
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