Anti-Aspergillus fumigatus [1E2] Antibody

This hybridoma line constitutively produces a mouse anti-Aspergillus fumigatus monoclonal antibody (clone 1E2) specific to the apical region of hyphae.

Aspergillus fumigatus is a widespread saprobe that breaks down organic material in soil. The conidia of this fungus are ubiquitous in the atmosphere and commonly inhaled. In healthy individuals, A. fumigatus can cause allergic reactions or localized infections. In the immunocompromised, however, it can cause invasive aspergillosis, an increasingly frequent and often fatal diease.

From the laboratory of Michelle Momany, PhD, University of Georgia

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA103
Anti-Aspergillus fumigatus [1E2] Hybridoma, 1 vial
1 vial 4-6 weeks
Regular Price:$913.00
On Sale:
EGA118
Anti-Aspergillus fumigatus [1E2] Antibody (supernatant), 5mL
5mL (supernatant) 4-6 weeks
Regular Price:$269.00
On Sale:
Specifications

Product Type: Antibody
Cell Type: Hybridoma cell line
Antigen: A. fumigatus cell wall
Isotype: IgM
Clonality: Monoclonal
Clone Name: 1 E2
Reactivity: Aspergillus fumigatus
Immunogen: A. fumigatus237 isolated cell walls
Growth Conditions: IMDM + 20% FBS + 1% Pen/Strep
Cryopreservation: Resuspend cells in 90% MDM, 10% DMSO. Store vials at -80C for 24-48 hrs at -70 before transfer to liquid nitrogen.
Species Immunized: Mouse
Epitope: Apical, no septa
Tested Applications: ELISA , IF 1:10
Storage: Liquid nitrogen
Shipped: Dry ice

Data

A. fumigatus mAbs show distinct localization patterns

A. fumigatus 237 was grown for 12–14 h, fixed, incubated with primary mAb, followed by FITC-conjugated anti-mouse secondary antibody, Calcofluor White to label chitin and Hoechst 33258 to label nuclei. Primary antibodies: mAb1E2 (a), mAb8A1 (b) and mAb19H1 (c). Left column, FITC; right column, chitinous cell wall and nuclei. Arrows mark conidia; arrowheads mark septa; the double arrowhead marks a newly formed branch from main hypha.

Adapted from: Momany, M., et al. (2004) Microbiology 150, 3261-8.

Provider
From the laboratory of Michelle Momany, PhD, University of Georgia
References
  1. Ste-Marie L, Sénéchal S, Boushira M, Garzon S, Strykowski H, Pedneault L, de Repentigny L. Production and characterization of monoclonal antibodies to cell wall antigens of Aspergillus fumigatus. Infect Immun. 1990 Jul;58(7):2105-14.
  2. Momany M, Lindsey R, Hill TW, Richardson EA, Momany C, Pedreira M, Guest GM, Fisher JF, Hessler RB, Roberts KA. The Aspergillus fumigatus cell wall is organized in domains that are remodelled during polarity establishment. Microbiology. 2004 Oct;150(Pt 10):3261-8.

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