These stable cell lines were derived from spontaneously arising tumors in the inbred VM/Dk mouse strain. When injected into VM/Dk mice, the cells lead to systemic metastasis with 100% fidelity. The cells express properties of macrophages or macrophage-like cells, similar to those reported previously in several types of human metastatic cancers. This VM tumor model is useful for evaluating potential therapies for managing metastatic tumors.
Available Cell Types:
Metastasis is the primary cause of morbidity and mortality for cancer patients and remains largely unmanageable. The failure to develop effective anti-metastatic drugs has been due in large part to the absence of in vivo metastatic tumor models that represent the full spectrum of metastatic disease.
From the laboratory of Thomas N. Seyfried, PhD, Boston College.
Part of The Investigator's Annexe program.
|Product Type:||Cell Line|
|Name:||VM-M2/Fluc, VM-M3/Fluc, VM-NM1/Fluc|
|Cell Type:||VM-M2 & VM-M3: Malignant Histiocytoma, VM-NM1: Malignant Astrocytoma|
|Accession ID:||VM-M2/Fluc, CVCL_0P41; VM-M3/Fluc, CVCL_0P42; VM-NM1/Fluc, CVCL_0P43|
|Morphology:||VM-M2 & VM-M3: Mesenchymal, VM-NM1: Spindle shaped|
|Subculturing:||1x106cells into a T25 flask|
|Growth Conditions:||Standard DMEM|
|Cryopreservation:||Complete media with 10% DMSO|
|Storage:||Store at -180C|
In vitro morphology and in vivo growth and metastatic spread of Metastatic Histiocytoma Cell Lines
(A) Phagocytic behavior was assessed from merging (Merge) the fluorescence (F) images and the differential interference contrast (DIC) images. (B) Growth and metastatic spread of VM-M3/Fluc tumor with bioluminescence imaging of firefly luciferase. Tumors were implanted s.c and i.c.. Multiple metastases were detected in vivo following implantation.
Adapted from: Huysentruyt LC, et al. Int J Cancer. 2008 Jul 1;123(1):73-84.
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